The Twist Fast Hybridization Target Enrichment protocol generates target-enriched DNA libraries for sequencing on NGS systems. This protocol details the steps for a flexible hybridization that can require as little as 15 minutes but can be adjusted to suit your needs for optimum performance in the shortest amount of time possible.

A component of the Twist Target Enrichment for NGS workflow, this protocol is:

• Designed for single or multiplex hybridization reactions using either Twist fixed or custom panels; optional secondary panels (spike-ins) can also be added for additional content
• Optimized for use with Twist Library Preparation Kits
• Should only be performed with the reagents specified, or their equivalents