The Twist TrueAmp Polymerase Mix is a 2X ready-to-use mix that includes a hot-start enzyme, optimized buffer, dNTPs, and uniformity enhancers for efficient, high-fidelity, and unbiased amplification of next-generation sequencing (NGS) Libraries.  This Protocol will highlight how this polymerase is:

• Engineered for Accurate, Low-Bias Amplification by delivering high-fidelity, uniform amplification across even the toughest GC extremes—ideal for sensitive applications like cfDNA, FFPE, and rare variant detection.
• Built for Automation and Easy Setup with hot-start, master-mix design prevents degradation and supports reliable, room-temperature or on-deck assembly.
• Dependable Performance from High to Ultra-Low Input with optimized cycling guides ensure strong yield and consistent results across a wide range of DNA inputs, down to picogram levels.