Analyzing Twist Targeted Methylation Sequencing Data Using the Twist Human Methylome Panel

This document is meant to serve as a guideline for processing next-generation sequencing (NGS) data from the Twist NGS Methylation Detection workflow using the Twist Human Methylome panel.

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Methylation analysis pipelines for NGS data are based on several open source tools and packages. This collateral describes best practices for using the BWA-meth/MethylDackel workflow to generate high quality CpG calls from the Twist Human Methylome panel.

Covered in this Technical Note

How to align reads

Perform CpG calling

Generate QC metrics

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Analyzing Twist Targeted Methylation Sequencing Data Using the Twist Human Methylome Panel

Related Resources

Optimizing the Performance of the Twist Targeted Methylation Sequencing Workflow

Minimizing Off-Target Capture in Targeted Methyl-Seq Through Panel Design & Enhancer Reagents

Capabilities of the Twist NGS Methylation Detection System