How are my antibodies inspected for quality?

Twist inspects every antibody product for yield (μg), purity (%), and size (kDa) under reducing conditions. Each antibody will be inspected via LabChip (CE-SDS) to inspect purity and size of the individual heavy and light chains. Yields are quantified using A280 readings. Supernatant samples are quantified using a titer determination method. In the finalized documentation, each purified antibody will have reported volume, yield, and concentration values in addition to percent purity and heavy chain and light chain sizing. Supernatant documentation will provide antibody titer expression levels of either low, medium or high expression levels.


Still have questions? Contact Us

What delivery formats are available?

  • 96-deep well plates - custom plate maps available
  • 1.4 mL micronic tube racks
  • 2 mL tubes
  • 4 mL tubes
  • 10 mL tubes (for 8 mL supernatant only)

Still have questions? Contact Us

What elution volumes do you supply and what are the expected yields of 1 mL and 8 mL scales?

1 mL = ~220 uL volume, 15-300 µg

8 mL = ~750 uL volume, 15-3000 µg


Still have questions? Contact Us

What buffer options do you offer for buffer exchange?

We use commercially available PBS, please share catalog information with customer upon request:

  • PBS 7.4 Cat# 21-031-CM Corning
  • PBS 6.0 Cat# P1760 Teknova

Still have questions? Contact Us

How are my antibodies inspected for quality?

Twist inspects every antibody product for yield (μg), purity (%), and size (kDa) under reducing conditions. Each antibody will be inspected via LabChip (CE-SDS) to inspect purity and size of the individual heavy and light chains. Yields are quantified using A280 readings. Supernatant samples are quantified using a titer determination method. In the finalized documentation, each purified antibody will have reported volume, yield, and concentration values in addition to percent purity and heavy chain and light chain sizing. Supernatant documentation will provide antibody titer expression levels of either low, medium or high expression levels.


Still have questions? Contact Us

What delivery formats are available?

  • 96-deep well plates - custom plate maps available
  • 1.4 mL micronic tube racks
  • 2 mL tubes
  • 4 mL tubes
  • 10 mL tubes (for 8 mL supernatant only)

Still have questions? Contact Us

What elution volumes do you supply and what are the expected yields of 1 mL and 8 mL scales?

1 mL = ~220 uL volume, 15-300 µg

8 mL = ~750 uL volume, 15-3000 µg


Still have questions? Contact Us

What buffer options do you offer for buffer exchange?

We use commercially available PBS, please share catalog information with customer upon request:

  • PBS 7.4 Cat# 21-031-CM Corning
  • PBS 6.0 Cat# P1760 Teknova

Still have questions? Contact Us