Shipping and Storage

Resuspension Guidelines:

• For resuspension, briefly centrifuge the tube before opening and resuspend in nuclease free Tris-EDTA (TE) buffer, pH 8.0 or 10 mM Tris-HCl, pH 8.0 to the desired concentration.
• A concentration of at least 10 ng/μL is recommended for the stock dilution, but the optimal concentration will need to be determined based on your desired application.
• Prepare aliquots of the stock dilution and separate working aliquots to limit chances of contamination and to reduce the number of freeze/thaw cycles. Use working aliquots as soon as possible after preparation and minimize exposure to high temperatures.

Resuspended DNA

• Twist recommends storing Resuspended DNA for the following durations based on storage condition.
  • Room Temperature for 3 months.
  • 4° C for 12 months.
  • -20° C for 24 months.
  • -80° C for long term storage.

Multiplexed Gene Fragments are provided as amplified double-stranded DNA, as a lyophilized product pooled in a single tube

1. Unmodified multiplexed gene fragments are stable. For long-term storage (up to one year), freeze DNA at -20° C. For even longer storage, freeze DNA at -80° C.
2. If the fragments are stored in solution, avoid repeated freeze-thaw cycles as this process can lead to physical degradation of the fragments.
3. If numerous experiments are planned, aliquot the sample and store at -20°C.
4. Careful handling is recommended to avoid possible contamination with nucleases or bacteria.

Twist Bioscience ships Multiplexed Gene Fragments dried down in tubes at ambient temperature. Double-stranded DNA is stable under most standard laboratory storage conditions. However, it is important to consider the following best practices to maintain the high quality of the DNA synthesized by Twist Bioscience.

• Twist recommends storing Dried DNA for the following durations based on storage condition.
  • Room Temperature for 3 months.
  • 4° C for 12 months.
  • -20° C for 24 months.
  • -80° C for long term storage.

Resuspension Guidelines:

• For resuspension, briefly centrifuge the tube before opening and resuspend in nuclease free Tris-EDTA (TE) buffer, pH 8.0 or 10 mM Tris-HCl, pH 8.0 to the desired concentration.
• A concentration of at least 10 ng/μL is recommended for the stock dilution, but the optimal concentration will need to be determined based on your desired application.
• Prepare aliquots of the stock dilution and separate working aliquots to limit chances of contamination and to reduce the number of freeze/thaw cycles. Use working aliquots as soon as possible after preparation and minimize exposure to high temperatures.

Resuspended DNA

• Twist recommends storing Resuspended DNA for the following durations based on storage condition.
  • Room Temperature for 3 months.
  • 4° C for 12 months.
  • -20° C for 24 months.
  • -80° C for long term storage.

Multiplexed Gene Fragments are provided as amplified double-stranded DNA, as a lyophilized product pooled in a single tube

1. Unmodified multiplexed gene fragments are stable. For long-term storage (up to one year), freeze DNA at -20° C. For even longer storage, freeze DNA at -80° C.
2. If the fragments are stored in solution, avoid repeated freeze-thaw cycles as this process can lead to physical degradation of the fragments.
3. If numerous experiments are planned, aliquot the sample and store at -20°C.
4. Careful handling is recommended to avoid possible contamination with nucleases or bacteria.

Twist Bioscience ships Multiplexed Gene Fragments dried down in tubes at ambient temperature. Double-stranded DNA is stable under most standard laboratory storage conditions. However, it is important to consider the following best practices to maintain the high quality of the DNA synthesized by Twist Bioscience.

• Twist recommends storing Dried DNA for the following durations based on storage condition.
  • Room Temperature for 3 months.
  • 4° C for 12 months.
  • -20° C for 24 months.
  • -80° C for long term storage.