Preparing Your Order

Pricing depends on the complexity tier (Pool Size) and the oligo length tier (20-120nt, 121-150nt, 151-200nt, 201-250nt, 251-300nt) of the oligo pool.

Pricing for oligo pools can be found on our website under your account.

If you need to contact your Account Manager regarding pricing, their contact information is available in eCommerce under Account.

Currently, our maximum oligo length is 300 bp.

Twist can synthesize oligos from 20 nucleotides up to a maximum of 300 nucleotides.  

Twist currently does not offer modified oligos.

The turnaround time for oligo pools is 2-4 business days*

*Twist Internal Data 2025. Time frame of 2-4 business days for Twist Oligo Pools up to 300 nt is based on internal data available as of 2025. This timeframe refers to the typical processing and handling time within our facilities before your order is handed over to the shipping carrier. Actual delivery times will vary depending on your location, the chosen shipping method, and the carrier's handling procedures.

Currently, the minimum length is 20 nucleotides for an oligo in an oligo pool order.

As of December 2025, Twist supports the use of the N degenerate base in Oligo Pools.

Customers may now incorporate N at specified positions within their oligo designs. At this time, other degenerate bases (R, Y, S, W, K, M, B, D, H, V) are not supported. If you wish to include a defined subset of bases at a specific position, this can be achieved by designing separate oligos, each containing a different base at the position of interest.

Pricing depends on the complexity tier (Pool Size) and the oligo length tier (20-120nt, 121-150nt, 151-200nt, 201-250nt, 251-300nt) of the oligo pool.

Pricing for oligo pools can be found on our website under your account.

If you need to contact your Account Manager regarding pricing, their contact information is available in eCommerce under Account.

Currently, our maximum oligo length is 300 bp.

Twist can synthesize oligos from 20 nucleotides up to a maximum of 300 nucleotides.  

Twist currently does not offer modified oligos.

The turnaround time for oligo pools is 2-4 business days*

*Twist Internal Data 2025. Time frame of 2-4 business days for Twist Oligo Pools up to 300 nt is based on internal data available as of 2025. This timeframe refers to the typical processing and handling time within our facilities before your order is handed over to the shipping carrier. Actual delivery times will vary depending on your location, the chosen shipping method, and the carrier's handling procedures.

Currently, the minimum length is 20 nucleotides for an oligo in an oligo pool order.

As of December 2025, Twist supports the use of the N degenerate base in Oligo Pools.

Customers may now incorporate N at specified positions within their oligo designs. At this time, other degenerate bases (R, Y, S, W, K, M, B, D, H, V) are not supported. If you wish to include a defined subset of bases at a specific position, this can be achieved by designing separate oligos, each containing a different base at the position of interest.