The Power of Silicon-based DNA Synthesis
Twist の高精度シリコンベース DNA合成プラットフォームによって、より高品質な遺伝子が得られ、スループットとスケーラビリティが大きく向上します。We offer:
Gene Fragments （二本鎖 DNA 断片）
Linear synthesized DNA sequences for direct cloning or larger gene assembly.
Clonal Genes （プラスミド DNA）
Synthesized DNA cloned into a plasmid vector and NGS sequence verified.
Reduce Costs With High Quality
Comparison of total costs for DNA synthesis, cloning, and sequence verification of constructs built with gene fragments. Cloning cost is assumed to be $30 per gene and sequencing cost is assumed to be $5 per clone sequenced. Sequencing more clones of fragments synthesized by the competitor is required to obtain correct sequence with 99% confidence, increasing the total cost for each gene.
High Quality Genes
Twist’s DNA synthesis technology exhibits extremely low error rates, the average error rate is one in 3,000 base pairs. その精度によって、より大きな遺伝子アセンブリの信頼性が高まり、その実施がより容易になります。See how our high quality DNA can accelerate your timelines here.
Simple Ordering Flexible Offering
Twist の人工遺伝子は、お客様のお望み通りの DNA を、ご希望の方法で柔軟にご提供します。お客様の視野を広げ、設計の幅を拡大し、発見を加速させます。
仕様
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.
お客様の声
おすすめの資料
アプリケーションノート
ウェビナー
Ordering online just got easier. Watch our “How to” video to see how to order genes all online from our eCommerce platform
The Power of Silicon-based DNA Synthesis
Twist の高精度シリコンベース DNA合成プラットフォームによって、より高品質な遺伝子が得られ、スループットとスケーラビリティが大きく向上します。We offer:
Gene Fragments （二本鎖 DNA 断片）
Linear synthesized DNA sequences for direct cloning or larger gene assembly.
Clonal Genes （プラスミド DNA）
Synthesized DNA cloned into a plasmid vector and NGS sequence verified.
Reduce Costs With High Quality
Comparison of total costs for DNA synthesis, cloning, and sequence verification of constructs built with gene fragments. Cloning cost is assumed to be $30 per gene and sequencing cost is assumed to be $5 per clone sequenced. Sequencing more clones of fragments synthesized by the competitor is required to obtain correct sequence with 99% confidence, increasing the total cost for each gene.
High Quality Genes
Twist’s DNA synthesis technology exhibits extremely low error rates, the average error rate is one in 3,000 base pairs. その精度によって、より大きな遺伝子アセンブリの信頼性が高まり、その実施がより容易になります。See how our high quality DNA can accelerate your timelines here.
Simple Ordering Flexible Offering
Twist の人工遺伝子は、お客様のお望み通りの DNA を、ご希望の方法で柔軟にご提供します。お客様の視野を広げ、設計の幅を拡大し、発見を加速させます。
仕様
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
お客様の声
おすすめの資料
Ordering online just got easier. Watch our “How to” video to see how to order genes all online from our eCommerce platform
Clonal Genes （プラスミド DNA）
Twist Bioscience’s platform is capable of synthesizing hundreds of thousands of genes each month to meet all your DNA needs. Our silicon-based platform for DNA synthesis enables us to create at scale, highly precise, sequence perfect clonal genes of various lengths and difficulty that are all NGS verified and delivered on-time when you need them.
完璧な遺伝子を配列解析
Clonal Genes のそれぞれに対して実施される、 NGS を用いた当社の標準的な検証結果例について、図に示しています。この図の Clonal Genes はエラーのないクローンの一例です。読み取り深度を見るとプラスミド全体にわたってリードが得られていることが分かり、SNP やインデルは検出されませんでした。
Clonal Genes の成功率
Twist Bioscience の Clonal Genes の成功率を図に示しています。成功率は、ご注文いただいた Clonal Genes のうち、SNP やインデルを含まずに納品された割合として定義しています。
FAQ
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Clonal Genes （プラスミド DNA）
Twist Bioscience’s platform is capable of synthesizing hundreds of thousands of genes each month to meet all your DNA needs. Our silicon-based platform for DNA synthesis enables us to create at scale, highly precise, sequence perfect clonal genes of various lengths and difficulty that are all NGS verified and delivered on-time when you need them.
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
完璧な遺伝子を配列解析
Clonal Genes のそれぞれに対して実施される、 NGS を用いた当社の標準的な検証結果例について、図に示しています。この図の Clonal Genes はエラーのないクローンの一例です。読み取り深度を見るとプラスミド全体にわたってリードが得られていることが分かり、SNP やインデルは検出されませんでした。
Clonal Genes の成功率
Twist Bioscience の Clonal Genes の成功率を図に示しています。成功率は、ご注文いただいた Clonal Genes のうち、SNP やインデルを含まずに納品された割合として定義しています。
FAQ
Twist’s DNA synthesis technology outperforms the competitions with exceptionally low error rates.
In a direct comparison of Gene Fragment products, Twist consistently showed the lowest error rate. The graph to the left represents the average error rate for Gene sequences ordered from Twist and Integrated DNA Technologies, Inc. (IDT). The results show Twist Gene Fragments have greater sequence accuracy over eBlocks and gBlocks with an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.
More perfect Genes from Twist
Twist Gene Fragments consistently yield the highest percentage of perfect clones which saves you time and money in your research. The graph to the left shows a direct comparison of the percentage of sequence perfect clones across a wide variety of gene lengths and sequences for 3 different gene products. Data was generated using a set of 63 sequences with a wide variety of gene lengths to reflect the diversity of gene lengths required for various real-world synthetic biology applications.
FAQ
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
Twist’s DNA synthesis technology outperforms the competitions with exceptionally low error rates.
In a direct comparison of Gene Fragment products, Twist consistently showed the lowest error rate. The graph to the left represents the average error rate for Gene sequences ordered from Twist and Integrated DNA Technologies, Inc. (IDT). The results show Twist Gene Fragments have greater sequence accuracy over eBlocks and gBlocks with an average of 2-fold greater accuracy in sequence fidelity for Twist Gene Fragments over gBlocks.
More perfect Genes from Twist
Twist Gene Fragments consistently yield the highest percentage of perfect clones which saves you time and money in your research. The graph to the left shows a direct comparison of the percentage of sequence perfect clones across a wide variety of gene lengths and sequences for 3 different gene products. Data was generated using a set of 63 sequences with a wide variety of gene lengths to reflect the diversity of gene lengths required for various real-world synthetic biology applications.
Learn how scientists are using Twist Genes in the Pandemic Protection Platform program, an accelerated antibody development process that aims to provide access to treatments within 60 days.
FAQ
製品シート
Clonal Genes 製品シート
Twist Bioscience は、合成生物学や分子生物学の中核をなす遺伝子合成に変革をもたらしています。当社のシリコンベース DNA 書き込みプラットフォームは、遺伝子合成のスループットとスケーラビリティを大きく向上させるだけでなく、納期を短縮し、１塩基あたりの価格も低減します。
製品シート
Gene Fragments 製品シート
Twist Bioscience の Gene Fragments は、コロニースクリーニングを最小限に抑えることで、クローニングプロセスを改善します。これにより、クローニングとシーケンシングのコストを劇的に削減でき、時間とコストの節約になります。
ケーススタディ
より良い DNA アセンブリラインの構築
Arzeda は、Labcyte、TeselaGen、Twist Bioscience の技術を活用し、合成生物学のワークフローを加速させています。
アプリケーションノート
5 kb の遺伝子合成によるパスウェイ・エンジニアリング
代謝工学のアプリケーションでは、研究者が微生物のリプログラミングを行って目的の非天然分子を生産するために、迅速な設計・構築・試験のサイクルが不可欠です。そうした分子を生産するため、研究者は通常、宿主生物に１つの遺伝子または遺伝子の経路全体を組み込みます。これらの遺伝子は、微生物宿主で発現させると共通の基質を目的分子に変換する、タンパク質や酵素をコードしています。
ケーススタディ
感染症に対する抗体開発の迅速化
DARPA から、医療従事者が現場でウイルス性疾患に立ち向かうための迅速な対応策の開発という野心的な目標を与えられたヴァンダービルト大学メディカルセンターは、すでに抗体の開発期間を大幅に短縮することに成功しました。Twist Bioscience のハイスループット人工遺伝子によって、ラボではこのプロセスを迅速化することができたのです。
ウェビナー
Amyris が株最適化のための次世代プラットフォームについて語る
このウェビナーで、 Twist の人工遺伝子がこの革新的企業による微生物工学の取り組みにどのように貢献しているかをご覧ください。https://www.twistbioscience.com/genes_bioengineeringwebinar_sunilchandran
製品シート
Twist Vectors 製品シート
Twist Bioscience は、 NGS で検証済みの高品質カスタム遺伝子を、他の方法では実現できないコストと規模で合成しています。人工遺伝子の複製や、発現研究に利用したいという研究者のために、 Twist は便利なオンライン注文プラットフォームを通して、さまざまなクローニングベクターや発現ベクターからご選択いただける納品オプションをご用意しています。
アプリケーションノート
Twist 人工遺伝子に関するアドバイス：人工遺伝子の設計方法
Twist Bioscience は、シリコンベース DNA合成プラットフォームを用いて高品質の人工 DNA を作製しています。Twist Bioscience のオンライン注文プラットフォームをお使いいただくことで、簡単に遺伝子の設計・注文が可能です。本書では、 Twist の Clonal Genes および Gene Fragments を、さまざまなアプリケーション向けに最適化しカスタマイズするためのヒントをご紹介します。
アプリケーションノート
Twist 人工遺伝子に関するアドバイス：カスタムベクターにおける人工遺伝子の最小鎖長要件を満たす
合成したい配列を送信すると、スコアリングアルゴリズムがその配列をチェックして合成可能かどうかを判断します。その際に最初にチェックされるのが、その配列が 300 bp 以上という鎖長要件を満たしているかどうかです。この最低鎖長要件を満たさない場合、遺伝子の設計に本文書をご活用ください。
アプリケーションノート
Gene Fragments クローニングガイドライン
Twist Bioscience の Gene Fragments は、長さ 300 〜 1800 塩基対の二本鎖 DNA です。この高品質な遺伝子断片には、5' 末端、3' 末端にアダプター配列が付加されています。
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
製品シート
Clonal Genes 製品シート
Twist Bioscience は、合成生物学や分子生物学の中核をなす遺伝子合成に変革をもたらしています。当社のシリコンベース DNA 書き込みプラットフォームは、遺伝子合成のスループットとスケーラビリティを大きく向上させるだけでなく、納期を短縮し、１塩基あたりの価格も低減します。
製品シート
Gene Fragments 製品シート
Twist Bioscience の Gene Fragments は、コロニースクリーニングを最小限に抑えることで、クローニングプロセスを改善します。これにより、クローニングとシーケンシングのコストを劇的に削減でき、時間とコストの節約になります。
ケーススタディ
より良い DNA アセンブリラインの構築
Arzeda は、Labcyte、TeselaGen、Twist Bioscience の技術を活用し、合成生物学のワークフローを加速させています。
アプリケーションノート
5 kb の遺伝子合成によるパスウェイ・エンジニアリング
代謝工学のアプリケーションでは、研究者が微生物のリプログラミングを行って目的の非天然分子を生産するために、迅速な設計・構築・試験のサイクルが不可欠です。そうした分子を生産するため、研究者は通常、宿主生物に１つの遺伝子または遺伝子の経路全体を組み込みます。これらの遺伝子は、微生物宿主で発現させると共通の基質を目的分子に変換する、タンパク質や酵素をコードしています。
ケーススタディ
感染症に対する抗体開発の迅速化
DARPA から、医療従事者が現場でウイルス性疾患に立ち向かうための迅速な対応策の開発という野心的な目標を与えられたヴァンダービルト大学メディカルセンターは、すでに抗体の開発期間を大幅に短縮することに成功しました。Twist Bioscience のハイスループット人工遺伝子によって、ラボではこのプロセスを迅速化することができたのです。
ウェビナー
Amyris が株最適化のための次世代プラットフォームについて語る
このウェビナーで、 Twist の人工遺伝子がこの革新的企業による微生物工学の取り組みにどのように貢献しているかをご覧ください。https://www.twistbioscience.com/genes_bioengineeringwebinar_sunilchandran
製品シート
Twist Vectors 製品シート
Twist Bioscience は、 NGS で検証済みの高品質カスタム遺伝子を、他の方法では実現できないコストと規模で合成しています。人工遺伝子の複製や、発現研究に利用したいという研究者のために、 Twist は便利なオンライン注文プラットフォームを通して、さまざまなクローニングベクターや発現ベクターからご選択いただける納品オプションをご用意しています。
アプリケーションノート
Twist 人工遺伝子に関するアドバイス：人工遺伝子の設計方法
Twist Bioscience は、シリコンベース DNA合成プラットフォームを用いて高品質の人工 DNA を作製しています。Twist Bioscience のオンライン注文プラットフォームをお使いいただくことで、簡単に遺伝子の設計・注文が可能です。本書では、 Twist の Clonal Genes および Gene Fragments を、さまざまなアプリケーション向けに最適化しカスタマイズするためのヒントをご紹介します。
アプリケーションノート
Twist 人工遺伝子に関するアドバイス：カスタムベクターにおける人工遺伝子の最小鎖長要件を満たす
合成したい配列を送信すると、スコアリングアルゴリズムがその配列をチェックして合成可能かどうかを判断します。その際に最初にチェックされるのが、その配列が 300 bp 以上という鎖長要件を満たしているかどうかです。この最低鎖長要件を満たさない場合、遺伝子の設計に本文書をご活用ください。
アプリケーションノート
Gene Fragments クローニングガイドライン
Twist Bioscience の Gene Fragments は、長さ 300 〜 1800 塩基対の二本鎖 DNA です。この高品質な遺伝子断片には、5' 末端、3' 末端にアダプター配列が付加されています。
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
記事・論文
Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning
記事・論文
The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells
記事・論文
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
記事・論文
Machine Learning-Guided Prediction of Antigen-Reactive In Silico Clonotypes Based on Changes in Clonal Abundance through Bio-Panning
記事・論文
The LRRC8A:C Heteromeric Channel Is a cGAMP Transporter and the Dominant cGAMP Importer in Human Vasculature Cells
記事・論文
In situ readout of DNA barcodes and single base edits facilitated by in vitro transcription
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
一般
Clonal Genes （プラスミド DNA）
コドン最適化
配列設計
10μg – 100μg | 100μg – 1mg
**Savings based on comparison to competitor’s publicly listed pricing as of 2022 年 8 月 15 日. Turnaround times between Twist and competitors may vary.
Genes Pricing
PRICE OF ALL GENES
Total cost of all genes and additional settings
DELIVERY FORMAT
2ml/4ml Tubes (for >10 genes): $50.00 OR Plate: $0.00
ADDITIONAL COSTS
Shipping, handling and taxes
合計
Price for BP and cloning fees based on length (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | 1.8 — 3.2 kbp | 3.2 — 5.0 kbp | |
|---|---|---|---|---|
| Price per bp | - | $0.09/bp | $0.13/bp | $0.18/bp |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| Cloning fee for: | ||||
| Twist Clonal Vector | $80.00/seq* | $35.00/seq | $35.00/seq | $35.00/seq |
| Custom Vector | $100.00/seq* | $55.00/seq | $55.00/seq | $55.00/seq |
| Twist Expression Vector | $120.00/seq* | $75.00/seq | $75.00/seq | $75.00/seq |
* 0.3 - 0.5 kbp genes have a per-construct price which includes Synthesis & Cloning.
DNA scale and settings prices (USD)
| 50 ng — 2 µg | 2 µg — 10 µg | 10 µg — 100 µg | 100 µg — 1 mg | |
|---|---|---|---|---|
| Price per sequence | $0.00 | $10.00/seq | $100.00/seq | $150.00/seq |
| Glycerol Stock (per construct) | $10.00 | $10.00 | $10.00 | $10.00 |
| dna grade | ||||
| Transfection Grade < 10 EU/µg | - | - | $0.00 | $0.00 |
| Endotoxin Free < 0.1 EU/µg | - | - | $50.00/seq | $50.00/seq |
| dna final state | ||||
| Normalization | - | - | $15.00/seq | $15.00/seq |
| Buffer type 1 - Water | - | - | $0.00 | $0.00 |
| Buffer type 2 - TE buffer low EDTA (10mM Tris-Cl pH 8.0, 0.1mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 3 - TE buffer (10mM Tris-Cl pH 8.0, 1 mM EDTA) | - | - | $0.00 | $0.00 |
| Buffer type 4 - Elution buffer (2 mM Tris-Cl, pH 8.5) | - | - | $0.00 | $0.00 |
Price for BP (USD)
| 0.3 — 0.5 kbp | 0.5 — 1.8 kbp | |||
|---|---|---|---|---|
| GENE FRAGMENTS （二本鎖 DNA 断片） | ||||
| With Adapters | $35.00/seq* | $0.07/bp | ||
| Price per bp | $40.00/seq* | $0.08/bp |
* 0.3 - 0.5 kbp genes have a per-sequence price which includes synthesis
DNA settings prices (USD)
| 50 ng — 2 µg | ||||
|---|---|---|---|---|
| NORMALIZATION | ||||
| Normalized to 250 ng per fragment | $10.00/seq | |||
| IN SOLUTION | ||||
| Elution buffer (25 µL) | $10.00/seq |
