Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers.
Twist Universal Adapters
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist 10 bp Unique Dual-Indexed (UDI) Primers
Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
Twist 12bp High Throughput (HT) UDI Primers
Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.
Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers.
Twist Universal Adapters
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist 10 bp Unique Dual-Indexed (UDI) Primers
Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
Twist 12bp High Throughput (HT) UDI Primers
Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.
Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”
右図では、 Twist Universal Adapters をインサートのライゲーションと UDI プライマーの PCR 増幅に使用することで、堅牢なライブラリを作製できることが示されています。
ライブラリ調製とターゲットエンリッチメントは、シーケンシングにおけるバリアントコールに影響を及ぼします。ハイブリッドキャプチャを成功させるには、一貫して堅牢なライブラリを構築することが重要です。ライブラリ調製時にアダプターが二量体化すると、望ましくない生成物が発生し、インサートへのライゲーションに利用できるアダプターの有効濃度が低下してしまいます。ライブラリ構築時に Twist Universal Adapters と Primers を使用することで、この問題を最小限に抑えることができます。
Twist Universal Adapters は、ライブラリ調製時のアダプターダイマー形成を最小限に抑えます。
Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”
右図では、 Twist Universal Adapters をインサートのライゲーションと UDI プライマーの PCR 増幅に使用することで、堅牢なライブラリを作製できることが示されています。
ライブラリ調製とターゲットエンリッチメントは、シーケンシングにおけるバリアントコールに影響を及ぼします。ハイブリッドキャプチャを成功させるには、一貫して堅牢なライブラリを構築することが重要です。ライブラリ調製時にアダプターが二量体化すると、望ましくない生成物が発生し、インサートへのライゲーションに利用できるアダプターの有効濃度が低下してしまいます。ライブラリ構築時に Twist Universal Adapters と Primers を使用することで、この問題を最小限に抑えることができます。
Twist Universal Adapters は、ライブラリ調製時のアダプターダイマー形成を最小限に抑えます。
101307
Twist Universal Adapter System TruSeq Compatible, 16 Samples101308
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate A101309
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate B101310
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate C101311
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate D106390
Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04106391
Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08106392
Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12106393
Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16106396
Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)106398
Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)106399
Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)106400
Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)106401
Twist HT Universal Adapter System TruSeq Comp, 384W AAA01106402
Twist HT Universal Adapter System TruSeq Comp, 384W AAA02106403
Twist HT Universal Adapter System TruSeq Comp, 384W AAA03106404
Twist HT Universal Adapter System TruSeq Comp, 384W AAA04106405
Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)106406
Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)106407
Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)106408
Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)The Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
101307
Twist Universal Adapter System TruSeq Compatible, 16 Samples101308
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate A101309
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate B101310
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate C101311
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate D106390
Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04106391
Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08106392
Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12106393
Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16106396
Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)106398
Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)106399
Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)106400
Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)106401
Twist HT Universal Adapter System TruSeq Comp, 384W AAA01106402
Twist HT Universal Adapter System TruSeq Comp, 384W AAA02106403
Twist HT Universal Adapter System TruSeq Comp, 384W AAA03106404
Twist HT Universal Adapter System TruSeq Comp, 384W AAA04106405
Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)106406
Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)106407
Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)106408
Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)The Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
安全データシート
Twist Universal Adapter System - TruSeq Compatible (Plate A), 96 Samples (SDS-001088)
101308用安全データシート
安全データシート
Twist Universal Adapter System - TruSeq Compatible (Plates B, C, and D), 96 Samples (SDS-001089)
Safety Data Sheet for 101309 - 101311
安全データシート
Twist Universal Adapter System - TruSeq Compatible, 16 Samples (SDS-001122)
101307用安全データシート
安全データシート
Twist HT UDI Primers - TruSeq Comp, Plate 96 Well Plates (SDS-001124)
Safety Data Sheets for 104493 - 10452
安全データシート
Twist HT UDI Primers - TruSeq Comp, 384 Well Plates (SDS-001123)
Safety Data Sheet for 104431-104438
安全データシート
Twist Universal Adapter System - TruSeq Compatible (Plate A), 96 Samples (SDS-001088)
101308用安全データシート
安全データシート
Twist Universal Adapter System - TruSeq Compatible (Plates B, C, and D), 96 Samples (SDS-001089)
Safety Data Sheet for 101309 - 101311
安全データシート
Twist Universal Adapter System - TruSeq Compatible, 16 Samples (SDS-001122)
101307用安全データシート
安全データシート
Twist HT UDI Primers - TruSeq Comp, Plate 96 Well Plates (SDS-001124)
Safety Data Sheets for 104493 - 10452
安全データシート
Twist HT UDI Primers - TruSeq Comp, 384 Well Plates (SDS-001123)
Safety Data Sheet for 104431-104438