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Bovine single-nucleotide polymorphism (SNP) genotyping panel for livestock genomics, breeding research, and trait analysis

Support livestock genomics research with a bovine SNP genotyping panel optimized for scalable sequencing and high-confidence variant analysis. Broad genomic coverage enables applications including genomic selection, breeding optimization, parentage testing, and agricultural biotechnology research while maintaining efficient sequencing performance.

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Optimized for livestock genomics

Supports genomic selection, breeding research, and agricultural biotechnology applications.

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Broad SNP coverage

Comprehensive genomic representation improves trait analysis and variant detection confidence.

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Efficient high-throughput workflow

Designed for scalable sequencing and streamlined bovine genotyping workflows.

Product data

The Twist FlexPrep UHT Library Preparation Kit workflow creates high-quality data for genotyping applications. Coupled with the Twist Genotyping Panel - Bovine 100k, you can capture sequencing reads on genomic regions of interest to achieve the required coverage for genotype calls. High genotype concordance is observed between the Genotyping Panel samples and the PCR-free ground truth whole genome sequencing (WGS).

The Twist Genotyping Panel - Bovine 100k is useful for genotype identification at target regions. For three example samples, genotypes were called for each SNP enriched by the panel. More than 75k SNPs were identified for each sample. Consistently, sample A was found to have more heterozygous alleles and fewer homozygous alternate alleles compared to samples B and C.

In an example region of chromosome 29, the higher heterozygous count in sample A is displayed at the TIGD3 and SCYL1 genes. Samples B and C have identical genotype calls at this region suggesting that these two individuals may be related and could have similar phenotypic traits. In addition, at the CDCA5 gene, sample A is homozygous for the reference allele and samples B and C are homozygous for the alternate allele. 

Video
Bovine targeted genotyping panel demonstrates high SNP and INDEL genotype concordance across sequencing downsampling levels compared with PCR-free reference data.
Genotype allele counts for bovine samples after target enrichment show distributions of homozygous reference, heterozygous, and homozygous alternate variants across replicate libraries.
Bovine chromosome 29 allele distribution map displays genotype variation across representative samples with annotated RefSeq transcripts and SNP allele classifications.

High-quality genotyping data

The Twist FlexPrep UHT Library Preparation Kit workflow creates high-quality data for genotyping applications. Coupled with the Twist Genotyping Panel - Bovine 100k, you can capture sequencing reads on genomic regions of interest to achieve the required coverage for genotype calls. High genotype concordance is observed between the Genotyping Panel samples and the PCR-free ground truth whole genome sequencing (WGS).

Bovine targeted genotyping panel demonstrates high SNP and INDEL genotype concordance across sequencing downsampling levels compared with PCR-free reference data.

The Twist Genotyping Panel - Bovine 100k libraries with various downsampling levels are compared to the PCR-free reference to calculate genotype concordance. Concordance is calculated for SNPs and INDELs independently. Data plotted represent sequencing from three samples, each processed with eight replicates.

Easily genotype your herd

The Twist Genotyping Panel - Bovine 100k is useful for genotype identification at target regions. For three example samples, genotypes were called for each SNP enriched by the panel. More than 75k SNPs were identified for each sample. Consistently, sample A was found to have more heterozygous alleles and fewer homozygous alternate alleles compared to samples B and C.

Genotype allele counts for bovine samples after target enrichment show distributions of homozygous reference, heterozygous, and homozygous alternate variants across replicate libraries.

Genotype counts by allele for three representative samples after library prep and target enrichment with 150X downsampling. Each sample was processed with eight replicates. Alleles are categorized as homozygous reference (hom ref), heterozygous (het), or homozygous alternate (hom alt).

Identify novel regions of interest

In an example region of chromosome 29, the higher heterozygous count in sample A is displayed at the TIGD3 and SCYL1 genes. Samples B and C have identical genotype calls at this region suggesting that these two individuals may be related and could have similar phenotypic traits. In addition, at the CDCA5 gene, sample A is homozygous for the reference allele and samples B and C are homozygous for the alternate allele. 

Bovine chromosome 29 allele distribution map displays genotype variation across representative samples with annotated RefSeq transcripts and SNP allele classifications.

Allele distribution for three samples on an example region of Bovine chromosome 29. Subset of annotated transcripts from NCBI RefSeq is displayed at the bottom. The color of the bar indicates the genotype of each sample. Alleles are categorized as homozygous reference (hom ref), heterozygous (het), or homozygous alternate (hom alt).

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Bovine targeted genotyping panel demonstrates high SNP and INDEL genotype concordance across sequencing downsampling levels compared with PCR-free reference data.

High-quality genotyping data

The Twist FlexPrep UHT Library Preparation Kit workflow creates high-quality data for genotyping applications. Coupled with the Twist Genotyping Panel - Bovine 100k, you can capture sequencing reads on genomic regions of interest to achieve the required coverage for genotype calls. High genotype concordance is observed between the Genotyping Panel samples and the PCR-free ground truth whole genome sequencing (WGS).

Genotype allele counts for bovine samples after target enrichment show distributions of homozygous reference, heterozygous, and homozygous alternate variants across replicate libraries.

Easily genotype your herd

The Twist Genotyping Panel - Bovine 100k is useful for genotype identification at target regions. For three example samples, genotypes were called for each SNP enriched by the panel. More than 75k SNPs were identified for each sample. Consistently, sample A was found to have more heterozygous alleles and fewer homozygous alternate alleles compared to samples B and C.

Bovine chromosome 29 allele distribution map displays genotype variation across representative samples with annotated RefSeq transcripts and SNP allele classifications.

Identify novel regions of interest

In an example region of chromosome 29, the higher heterozygous count in sample A is displayed at the TIGD3 and SCYL1 genes. Samples B and C have identical genotype calls at this region suggesting that these two individuals may be related and could have similar phenotypic traits. In addition, at the CDCA5 gene, sample A is homozygous for the reference allele and samples B and C are homozygous for the alternate allele.