Twist RNA Sequencing Workflow Solutions

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RNA-Seq Library Preparation Overview

Twist RNA library preparation offers workflow which was optimized to take a sample through to completed library in as little as 5 hours (left). The whole transcriptome libraries can be sequenced directly for comprehensive analysis. They may also be used as input into Twist RNA target enrichment workflow with our Target Enrichment panels for RNA Exome, RNA Fusion, or custom designed RNA enrichment panels which focus NGS coverage on coding regions enabling gene expression analysis, fusion detection, and identification of coding variants from a single assay (right).

RNA-Seq Library Preparation Workflow

Starting with total RNA as input from 1 ng up to 1 ug of input can be introduced into the workflow with or without the optional ribosomal RNA and Globin depletion module to remove unwanted RNA species. The RNA is fragmented and then reverse transcribed into cDNA followed by a single-step 2nd strand synthesis and A-tailing that saves time and procedure steps. Finally, libraries are constructed by ligating Twist high quality UMIs or Universal Adaptors (UDIs) followed by amplification.

RNA LP Product Sheet

This product sheet highlights the Twist RNA Exome's capabilities, providing essential information for superior genomic sequencing outcomes.

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RNA LP with UMI Protocol

This protocol details the steps to build sequencing libraries for target enrichment including converting RNA and preparing cDNA libraries with Twist UMIs.

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RNA LP with UDI Protocol

Protocol for RNA library preparation using universal adapter system.

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RNA Exome

The Twist RNA Exome, along with Twist RNA Library Prep and Twist Target Enrichment, provides a reliable tool for generating transcriptome sequencing data from RNA extracted from a variety of sources including FFPE. RNA Exome increases the signal while requiring fewer sequencing reads for the same number of genes detect. This enables the detection of low expressing targets that are critical for an accurate picture of the transcriptional state of the biological sample. Additionally, the RNA Exome’s exon-aware design approach leaves open the ability to detect isoforms and junctions that may otherwise get lost with typical design approaches. This complete sequencing solution can produce highly complex and uniform sequencing reads for RNA sequencing analysis of the protein coding sequences of the human transcriptome.

Designed for the Human Transcriptome

Target 35.8 Mb bases, 19,708 genes and 63,215 isoforms
Built with sequences from Gencode and RefSeq
More than 1.8-fold enrichment over whole transcriptome

Target Enrichment Built for RNA

Exon-aware probe design for protein coding regions, fusions and isoforms
Fewer reads per sample for more samples
Compatible with FFPE and low RNA input

More Targets, Fewer Reads

Twist RNA Exome detects more targets with fewer reads over a range of RNA inputs with FFPE and universal human reference RNA. All libraries were prepared with the Twist RNA Library Prep

RNA LP Product Sheet

This product sheet highlights the Twist RNA Exome's capabilities, providing essential information for superior genomic sequencing outcomes.

Get Product Sheet

RNA LP with UMI Protocol

This protocol details the steps to build sequencing libraries for target enrichment including converting RNA and preparing cDNA libraries with Twist UMIs.

Get Protocol

RNA LP with UDI Protocol

Protocol for RNA library preparation using universal adapter system.

Get Protocol

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RNA Fusion

Gene fusions often act as oncogenic driver mutations in a wide variety of cancer types. Their rapid and accurate detection can inform clinical actions and drive treatment decisions. The Twist Alliance CeGaT RNA Fusion Panel Kit is an RNA-based enrichment panel and offers an improved targeted and sensitive approach to investigate gene fusions than the conventional methods.

This panel includes 160 fusion genes associated with approximately 30 cancer types. The fusion genes were selected by CeGaT, a leading genetic diagnostic and sequencing company located in Europe. The design is not only for the detection of known gene fusions but is also used to identify novel gene fusions.

The panel is optimized and validated for use with the Twist RNA Library Prep Kit for a complete end-to-end workflow exceptional performance. High capture efficiency with the RNA-based enrichment increases the sensitivity of detection. This panel is ideal for screening oncology samples to inform treatment options or discover novel fusions for tumor classifications.*