Engineered Performance for Reliable, Scalable cfDNA Workflows
Generate high quality libraries from challenging cfDNA inputs with a streamlined workflow that delivers high conversion and sensitivity for rare variant detection. Designed for low input and fragmented samples, the Twist cfDNA Library Preparation Kit helps you uncover critical insights with confidence and efficiency.
Maximize Rare Variant Detection
High conversion rate reliably detects rare variants (≤0.1% VAF). High coverage libraries compatible with duplex sequencing workflows supported by the Twist UMI Adapter System.
Streamline Workflow of Precious Samples
Robust performance, even with low (<1ng) sample input Sequence-ready libraries in 2 hours.
Amplification without Bias
Delivers consistent coverage across 5%–95% GC, preserving visibility of challenging genomic regions and difficult-to-capture targets. Consistent high yields from complex libraries —even at ≤100 pg DNA input. Call variants with confidence via improved fidelity and reduced homopolymer slippage
Engineered Performance for Reliable, Scalable cfDNA Workflows
Generate high quality libraries from challenging cfDNA inputs with a streamlined workflow that delivers high conversion and sensitivity for rare variant detection. Designed for low input and fragmented samples, the Twist cfDNA Library Preparation Kit helps you uncover critical insights with confidence and efficiency.
Maximize Rare Variant Detection
High conversion rate reliably detects rare variants (≤0.1% VAF). High coverage libraries compatible with duplex sequencing workflows supported by the Twist UMI Adapter System.
Streamline Workflow of Precious Samples
Robust performance, even with low (<1ng) sample input Sequence-ready libraries in 2 hours.
Amplification without Bias
Delivers consistent coverage across 5%–95% GC, preserving visibility of challenging genomic regions and difficult-to-capture targets. Consistent high yields from complex libraries —even at ≤100 pg DNA input. Call variants with confidence via improved fidelity and reduced homopolymer slippage
Product data
Figure 1. Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Preparation Kit was used to generate libraries with Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. The PCR condition was amplified using 6 cycles with Twist TrueAmp Polymerase mix and UDI primers. The libraries were pooled, sequenced and analyzed. Comparable error rates are seen between PCR-free and TrueAmp libraries.
Figure 2. Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
Figure 3. Twist cfDNA Library Preparation and Hyb Mix Kit enables sensitive and accurate detection of variants at 0.25% VAF. Libraries were single-plex captured with a custom 50kb oncology panel targeting 217 SNP variant sites in the Pan-Cancer standard material. Variants Missed is calculated by summing sites with less than 1 variant family divided by total detectable variant sites.
Figure 4. Twist cfDNA Library Preparation and Hyb Mix Kit delivers higher complexity with and without UMI deduplication.
Figure 5. Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields with native cfDNA samples. Following library generation, samples were eluted in 20 uL of water and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
cfDNA Library Prep Kit with TrueAmp Polymerase Mix Workflow
The cfDNA workflow leverages engineered GC balance and high fidelity amplification. This design delivers high amplification yield while minimizing PCR introduced errors that can compromise low frequency variant detection.
The result is uniform, high-quality libraries in a fast, 2-hour workflow. Aptamer-based hot-start control provides room-temperature stability for automated setup, while the single-tube 2× mastermix streamlines both WGS and Target Enrichment workflows.
Product data
Figure 1. Comparable Base Substitution Error Rates with and without TrueAmp Amplification. The Twist cfDNA Library Preparation Kit was used to generate libraries with Twist cfDNA Pan-Cancer Reference Standard v2 as the input material. Twist Full Length Unique Dual Index (UDI) Adapters were used during ligation for the PCR-free condition and Twist Universal Adapters were used during ligation for the PCR condition. The PCR condition was amplified using 6 cycles with Twist TrueAmp Polymerase mix and UDI primers. The libraries were pooled, sequenced and analyzed. Comparable error rates are seen between PCR-free and TrueAmp libraries.
Figure 2. Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields. Following library generation, samples were eluted in 20 uL of water and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
Figure 3. Twist cfDNA Library Preparation and Hyb Mix Kit enables sensitive and accurate detection of variants at 0.25% VAF. Libraries were single-plex captured with a custom 50kb oncology panel targeting 217 SNP variant sites in the Pan-Cancer standard material. Variants Missed is calculated by summing sites with less than 1 variant family divided by total detectable variant sites.
Figure 4. Twist cfDNA Library Preparation and Hyb Mix Kit delivers higher complexity with and without UMI deduplication.
Figure 5. Twist cfDNA Library Preparation Kit delivers higher cfDNA library yields with native cfDNA samples. Following library generation, samples were eluted in 20 uL of water and 1 uL was quantified with Qubit™ dsDNA Broad Range Kit.
cfDNA Library Prep Kit with TrueAmp Polymerase Mix Workflow
The cfDNA workflow leverages engineered GC balance and high fidelity amplification. This design delivers high amplification yield while minimizing PCR introduced errors that can compromise low frequency variant detection.
The result is uniform, high-quality libraries in a fast, 2-hour workflow. Aptamer-based hot-start control provides room-temperature stability for automated setup, while the single-tube 2× mastermix streamlines both WGS and Target Enrichment workflows.
Highlighted resources
Product Sheet
Protocol
Technical Note
Highlighted resources
Related Workflows and Applications
Target enrichment workflows with Twist deliver scalable, low bias, and highly uniform coverage across large and complex genomic regions; enabling broader discovery, higher sensitivity, and greater flexibility than amplicon or array based methods.
Twist offers several tools to support research into liquid biopsies and early cancer characterization.
Related Workflows and Applications
Target enrichment workflows with Twist deliver scalable, low bias, and highly uniform coverage across large and complex genomic regions; enabling broader discovery, higher sensitivity, and greater flexibility than amplicon or array based methods.
Twist offers several tools to support research into liquid biopsies and early cancer characterization.
