The Benefits of Enzymatic Conversion in Methyl-seq Workflows

While most methods rely on bisulfite conversion of DNA to detect unmethylated cytosines, bisulfite treatment requires high temperatures and a harsh pH that can damage DNA, which is particularly challenging with small sample size or already fragile sample types.

With the Twist Methylation Detection System, you can rescue DNA molecules and experience uniform genomic coverage of more DNA regions by using an enzymatic conversion method. This method allows you to detect up to 15% more methylated cytosines, increasing confidence in methylated-base calls for the study of epigenetics.

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Targeted Epigenome Sequencing of cfDNA Can Improve Sensitivity for Early Cancer Detection

Gahee Park, PhD, from the University of Cambridge shares how DNA methylation changes are robust cancer-specific markers, and how the Twist NGS Methylation Detection System helped her team improve enrichment accuracy three-fold, while decreasing experimental costs four-fold.

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Twist Long Oligos Power MPRAs

Our silicon platform allows us to quickly generate large, accurate and highly uniform MPRA libraries consisting of oligos up to 300 base pairs in length.

These libraries have many advantages:

• Accurate oligonucleotide synthesis allows you to have confidence in your results and trust that the DNA sequence you design is the sequence you’ll test
• High uniformity removes bias and screening burden from over and underrepresented elements
• Access to long oligonucleotide libraries means barcodes can be implemented without sacrificing the sequence of interest
• Long oligonucleotides also mean more of the natural genetic context can be encoded—enabling more biologically accurate analyses