Discover Antibodies Against Challenging Glycan Antigens
Discover Antibodies Against Challenging Glycan Antigens
Discover Antibodies Against Challenging Glycan Antigens
OVERVIEW SPECIFICATIONS THE PROCESS RESOURCES
Overview

Unlock Carbohydrate Antibody Discovery

The Twist Carbohydrate scFv Library is a synthetic phage display antibody library that leverages diverse structural information from 130 carbohydrate-binding antibodies that bind a range of human (carcinoma), viral (Ebola and HIV), and bacterial (Cholera, Shigella, and Chlamydia) glycan antigens.

This unique library can empower your glycan-targeted drug discovery and development in important therapeutic areas including oncology, inflammation, and infectious diseases.

Produce robust scFv antibodies against glycans
Produce robust scFv antibodies against glycans
Proven, highly manufacturable framework
Fully human antibody sequences
2 x 10^9 diversity
Capitalize on proven ion channel binding motifs
Capitalize on proven ion channel binding motifs
Binding sites informed by 130 validated antibodies
Improved binding contacts with positively and negatively charged amino acids in CDR3
Synthetic library advantage
Synthetic library advantage
Panning to functional assays in 10-12 weeks without immunization
Focus on effective sequence space
Screen multiple targets simultaneously

Unlock Carbohydrate Antibody Discovery

The Twist Carbohydrate scFv Library is a synthetic phage display antibody library that leverages diverse structural information from 130 carbohydrate-binding antibodies that bind a range of human (carcinoma), viral (Ebola and HIV), and bacterial (Cholera, Shigella, and Chlamydia) glycan antigens.

This unique library can empower your glycan-targeted drug discovery and development in important therapeutic areas including oncology, inflammation, and infectious diseases.

Produce robust scFv antibodies against glycans
Produce robust scFv antibodies against glycans
Proven, highly manufacturable framework
Fully human antibody sequences
2 x 10^9 diversity
Capitalize on proven ion channel binding motifs
Capitalize on proven ion channel binding motifs
Binding sites informed by 130 validated antibodies
Improved binding contacts with positively and negatively charged amino acids in CDR3
Synthetic library advantage
Synthetic library advantage
Panning to functional assays in 10-12 weeks without immunization
Focus on effective sequence space
Screen multiple targets simultaneously
Specifications
Library Specifications

The Twist Carbohydrate scFv Library combines heavy chain (VH) and light chain (VL) libraries to yield a fully human scFv library of 2 x 109 size. The heavy chain design shuffles 56 unique HCDR1s and 65 unique HCDR2s in the context of the human IGHV3-23 framework. The light chain design incorporates 62 unique LCDR1s and 47 unique LCDR2s in the context of the human IGKV4-1 framework. The CDR3 regions derive their diversity from 52 structures of antibodies in complex with carbohydrate antigens and are biased towards incorporating residues that make up the carbohydrate-antigen interface. These CDR3 regions include both positively and negatively charged amino acids, as observed in the 130 carbohydrate-binding antibodies from which this library is derived.

Heavy chain Design
Library Specifications

The Twist Carbohydrate scFv Library combines heavy chain (VH) and light chain (VL) libraries to yield a fully human scFv library of 2 x 109 size. The heavy chain design shuffles 56 unique HCDR1s and 65 unique HCDR2s in the context of the human IGHV3-23 framework. The light chain design incorporates 62 unique LCDR1s and 47 unique LCDR2s in the context of the human IGKV4-1 framework. The CDR3 regions derive their diversity from 52 structures of antibodies in complex with carbohydrate antigens and are biased towards incorporating residues that make up the carbohydrate-antigen interface. These CDR3 regions include both positively and negatively charged amino acids, as observed in the 130 carbohydrate-binding antibodies from which this library is derived.

Heavy chain Design
The Process
Library Panning & Screening Process

Go from panning to functional assays in 10-12 weeks. The process starts with phage screening the diverse Twist Carbohydrate scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.

You can also license the Carbohydrate scFv library to initiate your own in-house discovery projects. To learn more, get in touch at [email protected]

Library Planning and Screening
Library Panning & Screening Process

Go from panning to functional assays in 10-12 weeks. The process starts with phage screening the diverse Twist Carbohydrate scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.

You can also license the Carbohydrate scFv library to initiate your own in-house discovery projects. To learn more, get in touch at [email protected]

Library Planning and Screening
Resources