Leverage Insights from Crystallized Antibody Structures
Leverage Insights from Crystallized Antibody Structures
Leverage Insights from Crystallized Antibody Structures
OVERVIEW SPECIFICATIONS THE PROCESS PROOF OF CONCEPT RESOURCES
Overview

Harness Structural Data for Antibody Discovery

The Twist Structural scFv Library harnesses sequences from every antibody crystal structure in a worldwide protein structure database. By building on the knowledge of antibody structure-function relationships, this synthetic antibody library provides a platform for generating a wide range of therapeutic antibodies for any indication.

Produce robust scFv antibodies
Produce robust scFv antibodies
Proven, highly manufacturable framework with low immunogenicity and development liabilities removed
Fully human antibody sequences
4 x 10^10 diversity
Harness antibody crystal structures
Harness antibody crystal structures
Binding sites informed by 3,700 crystal structures
Uses structural information to confer superior binding affinity, specificity, and structural diversity
Synthetic library advantage
Synthetic library advantage
Panning to functional assays in 10-12 weeks without immunization
Focus on effective sequence space
Screen multiple targets simultaneously

Harness Structural Data for Antibody Discovery

The Twist Structural scFv Library harnesses sequences from every antibody crystal structure in a worldwide protein structure database. By building on the knowledge of antibody structure-function relationships, this synthetic antibody library provides a platform for generating a wide range of therapeutic antibodies for any indication.

Produce robust scFv antibodies
Produce robust scFv antibodies
Proven, highly manufacturable framework with low immunogenicity and development liabilities removed
Fully human antibody sequences
4 x 10^10 diversity
Harness antibody crystal structures
Harness antibody crystal structures
Binding sites informed by 3,700 crystal structures
Uses structural information to confer superior binding affinity, specificity, and structural diversity
Synthetic library advantage
Synthetic library advantage
Panning to functional assays in 10-12 weeks without immunization
Focus on effective sequence space
Screen multiple targets simultaneously
Specifications
Library Specifications

The Twist Structural scFv Library is a synthetic antibody library that incorporates the CDR sequences from 3,700 antibodies with known crystal structures from a protein structure database. To improve manufacturability, liabilities such as unpaired C- and N-glycosylation, deamination, and hydrolysis sites are eliminated. The heavy chain (VH) library shuffles 148 unique CDR1s, 151 unique CDR2s, and 564 unique CDR3s into the human IGHV3-23 framework. The light chain (VL) library shuffles 134 unique CDR1s, 158 unique CDR2s, and 278 unique CDR3s into the human IGKV1-39 framework. When combined, the VH and VL libraries yield a fully human scFv library with a diversity of 4 x 1010.

Heavy chain Design
Library Specifications

The Twist Structural scFv Library is a synthetic antibody library that incorporates the CDR sequences from 3,700 antibodies with known crystal structures from a protein structure database. To improve manufacturability, liabilities such as unpaired C- and N-glycosylation, deamination, and hydrolysis sites are eliminated. The heavy chain (VH) library shuffles 148 unique CDR1s, 151 unique CDR2s, and 564 unique CDR3s into the human IGHV3-23 framework. The light chain (VL) library shuffles 134 unique CDR1s, 158 unique CDR2s, and 278 unique CDR3s into the human IGKV1-39 framework. When combined, the VH and VL libraries yield a fully human scFv library with a diversity of 4 x 1010.

Heavy chain Design
The Process
Library Panning & Screening Process

Go from panning to functional assays in 10–12 weeks. The process starts with phage screening the diverse Twist Structural scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.

You can also license the Structural scFv Library to initiate your own in-house discovery projects. To learn more, get in touch at [email protected]

This Data Shows
Library Panning & Screening Process

Go from panning to functional assays in 10–12 weeks. The process starts with phage screening the diverse Twist Structural scFv Library against target antigens and ends with reformatting candidate antibody fragments to full-length IgG.

You can also license the Structural scFv Library to initiate your own in-house discovery projects. To learn more, get in touch at [email protected]

This Data Shows
Proof of Concept

Proof of Concept Data

The Twist Structural scFv Library was successfully panned against CD3, an important cell surface target in immunology, to identify unique clones, such as TB138-6, with desirable properties.

Cross-Reactive with Cynomolgus CD3

Titration ELISA shows the cross-reactivity of TB138-6 with human and cynomolgus monkey CD3.

Cross-Reactive with Cynomolgus CD3
Binds Cell Surface CD3 on Human CD8+ T-cells

Flow titration demonstrates that TB138-6 binds CD3+ cells (CD8+ T-cell) and not CD3- cells (CHO-GLP1R).

Binds Cell Surface CD3 on Human CD8+ T-cells

Proof of Concept Data

The Twist Structural scFv Library was successfully panned against CD3, an important cell surface target in immunology, to identify unique clones, such as TB138-6, with desirable properties.

Cross-Reactive with Cynomolgus CD3

Titration ELISA shows the cross-reactivity of TB138-6 with human and cynomolgus monkey CD3.

Cross-Reactive with Cynomolgus CD3
Binds Cell Surface CD3 on Human CD8+ T-cells

Flow titration demonstrates that TB138-6 binds CD3+ cells (CD8+ T-cell) and not CD3- cells (CHO-GLP1R).

Binds Cell Surface CD3 on Human CD8+ T-cells
Resources