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Compare agrigenomics solutions

NGS-based agrigenomics workflows provide greater flexibility, scalability, and genomic insight compared to traditional microarray platforms. With FlexPrep™, researchers can move beyond predefined variant sets and detect known and novel variants at base-pair resolution.

Features

NEXT GENERATION SEQUENCING WITH FLEXPREP

MICROARRAYS

Data completeness

Comprehensive, base-pair resolution across genome or selected regions

Limited to pre-selected variants; not well-suited for novel variant detection.

Customization of content

Easily customizable panels with fast TAT—target any genes, regions, or species

Content is fixed or semi-custom; change require redesign and new manufacturing

Cost and throughput

Lower cost per sample at scale due to multiplexing (up to 1,152 samples)

Cost scales poorly for large programs or across multiple species

Twist workflow for agrigenomics

The Twist agrigenomics workflow streamlines targeted sequencing from sample preparation through data generation, enabling efficient, scalable analysis for plant and animal genomics applications. Using FlexPrep™ library preparation and customizable target enrichment solutions, researchers can process large sample volumes with simplified workflows, high multiplexing capacity, and consistent performance across diverse species and sample types. The workflow is designed to support applications such as genotyping, trait selection, and population analysis while reducing hands-on time and sequencing costs.

1

Extraction

Twist DNA Purification Kit as part of FlexPrep UHT

2

Library Prep

FlexPrep UHT Library Prep Kit

3

Target Enrichment

Bovine Panel and Bovine Blocker Solution or Custom Panels

4

Sequencing

Element Biosciences, Ultima Genomics, or Illumina sequencing

5

Analysis

Curio Genomics, Khufu, or Gencove

Extraction

Twist DNA Purification Kit

The Twist DNA Purification Kit module streamlines sample prep by taking even challenging, “dirty” samples directly into FlexPrep library preparation. Built on an innovative single-wash extraction chemistry, the kit eliminates the traditional bind–wash–elute cycle—reducing hands-on time, consumable use, and workflow complexity.

Sold as a bundled workflow with FlexPrep, the Twist FlexPrep UHT Pure Ag DNA LP Kit simplifies DNA extraction without compromising quality.

Sequencing

Element Biosciences

By leveraging the Trinity Freestyle™ Hybridization Kit, FlexPrep maximizes the throughput capabilities of Element sequencers. This workflow compounds the time savings of FlexPrep’s easy workflow with the hands-off time afforded by on-sequencer Trinity enrichment chemistry.

Analysis

Curio Genomics

Scale your agrigenomics research with FlexPrep™ UHT Library Preparation Kit for low-pass sequencing and Curio Genomics for streamlined analysis. Twist's flexible library prep integrates seamlessly with Curio Genomics' platform, which offers high-throughput processing and efficient imputation, enabling cost-effective genotyping across large populations.

Gencove

Combining the FlexPrep™ UHT Library Preparation Kit with Gencove's imputation pipeline enables high-confidence variant calling from reduced sequencing coverage, maximizing efficiency and throughput and allowing you to generate valuable insights from genotyping data with exceptional accuracy and reduced sequencing costs.