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IVDR Solutions
Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
Streamline library preparation across T-A workflows
Specifically engineered to suppress the formation of adapter dimers, this system enables higher molecular complexity and reduced duplication for the full range of NGS applications.
Maximize usable library yield
Minimize the formation of adapter dimers for higher data quality and improved sequencing efficiency.
Accelerate sample preparation
Reduce the number of required PCR cycles via high ligation efficiency, for faster workflows and fewer amplification-induced errors.
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Enable scalable high-density multiplexing
Support cost-effective studies with 10-bp unique dual indexes (UDIs) and improved sample demultiplexing on all high-throughput sequencing platforms.
Product data
Repeatable performance ensures confidence in results. When experimental conditions are held constant, Twist Universal Adapters, combined with the Twist TrueAmp Library Prep Kit, produces a robust, consistent DNA library fragment size across a wide range of sample inputs.
Electropherograms of NGS libraries generated with the Twist TrueAmp Library Prep Kit. The overlap of the fluorescent curves demonstrates the consistency in library preparation that can be achieved from run to run. Samples were analyzed using the Agilent TapeStation D5000. 500 ng, 100 ng, 50 ng, 10 ng, and 1 ng were fragmented at 32°C. 3, 5, 6, 8, and 10 cycles of PCR were used for amplification, respectively. Samples have been performed in duplicates for each DNA input concentration.
Post-amplification library concentration across a range of DNA inputs highlights sufficient yield for downstream applications even when starting with as little as 1 ng.
Concentration of libraries after amplification for various DNA inputs. 500 ng, 100 ng, 50 ng, 10 ng, and 1 ng were fragmented at 32°C. 3, 5, 6, 8, and 10 cycles of PCR were used for amplification, respectively. Samples have been performed in duplicates for each DNA input concentration.
Video
Consistent library size across inputs as low as 1 ng
Repeatable performance ensures confidence in results. When experimental conditions are held constant, Twist Universal Adapters, combined with the Twist TrueAmp Library Prep Kit, produces a robust, consistent DNA library fragment size across a wide range of sample inputs.
Electropherograms of NGS libraries generated with the Twist TrueAmp Library Prep Kit. The overlap of the fluorescent curves demonstrates the consistency in library preparation that can be achieved from run to run. Samples were analyzed using the Agilent TapeStation D5000. 500 ng, 100 ng, 50 ng, 10 ng, and 1 ng were fragmented at 32°C. 3, 5, 6, 8, and 10 cycles of PCR were used for amplification, respectively. Samples have been performed in duplicates for each DNA input concentration.
Strong library yield after amplification across DNA inputs
Post-amplification library concentration across a range of DNA inputs highlights sufficient yield for downstream applications even when starting with as little as 1 ng.
Concentration of libraries after amplification for various DNA inputs. 500 ng, 100 ng, 50 ng, 10 ng, and 1 ng were fragmented at 32°C. 3, 5, 6, 8, and 10 cycles of PCR were used for amplification, respectively. Samples have been performed in duplicates for each DNA input concentration.
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Consistent library size across inputs as low as 1 ng
Repeatable performance ensures confidence in results. When experimental conditions are held constant, Twist Universal Adapters, combined with the Twist TrueAmp Library Prep Kit, produces a robust, consistent DNA library fragment size across a wide range of sample inputs.
Strong library yield after amplification across DNA inputs
Post-amplification library concentration across a range of DNA inputs highlights sufficient yield for downstream applications even when starting with as little as 1 ng.
Which adapter system is right for you?
Twist's Adapter Systems improve the performance of any 'T-A' overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. They reduce adapter dimer formation, increase yield, and can eliminate or minimize PCR—improving data quality while lowering sequencing costs. With broad compatibility, high multiplexing (up to 3,072 indexes), and ready-to-use formats, they scale effortlessly from discovery to high-throughput studies.
Universal Adapter System
HT Universal Adapter System
Full Length UDI Adapters
UMI Adapter System
Best for
Most applications
High Throughput applications
PCR-free Whole Genome Sequencing
Improved NGS variant accuracy
Contains
Universal Adapters; UDI Primers
Universal Adapters; HT UDI Primers
Full Length UDI Adapters
UMI Adapters; UDI Primers
Unique indexes
16 or 384
3072
1536
16 or 384
Index length
10 bp
12 bp
10 bp
10 bp
Available formats
96-well plates
96- or 384-well plates
96-well plates
96-well plates
Stay updated
Universal Adapter System
Best for
Most applications
Contains
Universal Adapters; UDI Primers
Unique indexes
16 or 384
Index length
10 bp
Available formats
96-well plates
HT Universal Adapter System
Best for
High Throughput applications
Contains
Universal Adapters; HT UDI Primers
Unique indexes
3072
Index length
12 bp
Available formats
96- or 384-well plates
Full Length UDI Adapters
High Throughput applications
Universal Adapters; HT UDI Primers
3072
12 bp
96- or 384-well plates
UMI Adapter System
PCR-free Whole Genome Sequencing
Full Length UDI Adapters
1536
10 bp
96-well plates
