Detecting cancer early and monitoring its progression depends on reliably sequencing cell-free DNA (cfDNA), but cfDNA is fragmented often in low-concentrations, and unforgiving of prep errors. Traditional error-correction approaches like UMIs require deep sequencing and complex bioinformatics, driving up cost and complexity. This application note shows how the Twist cfDNA Library Preparation Kit, paired with Ultima Genomics' ppmSeq adapter technology, enables accurate rare variant detection without PCR, target enrichment, or oversequencing. Twist kit's optimized enzyme chemistry maximizes conversion of fragmented DNA into high-quality libraries, while ppmSeq captures both strands of each DNA molecule in a single read, separating true mutations from sequencing errors at the read level. The result is cost-effective whole genome sequencing of cfDNA with the sensitivity needed for ultralow-frequency variant detection.

Download the application note to see the performance data.