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The Twist TrueAmp Library Preparation Kit provides the reagents needed to prepare genomic DNA (gDNA) libraries using enzymatic gDNA fragmentation and the Twist Universal Adapter System. The Twist Universal Adapter system consists of Twist Universal Adapters and Twist Unique Dual Indexed (UDI) Primers. This manual details the steps for generating the amplified, indexed libraries needed for downstream target enrichment and sequencing.