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Twist Bioscience HQ
681 Gateway Blvd
South San Francisco, CA 94080
DNA Resuspension and Storage Protocols
This document provides instructions for resuspending many of Twist’s synthetic biology products, including Clonal Genes, Gene Fragments, Gene Pools, Oligo Pools, and Variant Libraries.
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Twist Bioscience ships its DNA products dried down or resuspended in 2 mL microcentrifuge tubes, 96-well plates, or 384-well plates. Dried DNA is shipped at ambient temperature and resuspended DNA is shipped frozen.
Double-stranded DNA and single-stranded oligonucleotides are stable under most standard laboratory storage conditions. However, it is important to consider the following best practices to maintain the high quality of the DNA synthesized by Twist Bioscience.
Covered in this Protocol
Recommended resuspension buffer
Stock preparation and aliquoting recommendations
Stability and storage guidelines
Special instructions for low yield Clonal Genes
