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FAQ Home

Codon Optimization

  • Which organisms does Twist currently support for codon optimization?
  • If my gene is scored as Impossible, can I still have it synthesized?
  • For codon optimization, what steps are taken to maintain wild-type protein expression levels?

Sequence Design

  • Are there any sequence limitations/design guidelines for genes which I should follow?
  • What do the scoring results of my gene mean?
  • How do I convert my Amino Acid sequence to a nucleic acid sequence?
  • What lengths of synthetic DNA does Twist currently offer for synthesis?

General

  • Where can I find an SDS for gene products?
  • What is the shelf life of Gene Fragments and Clonal Genes?
  • How does Twist test the quality of the gene synthesis?
  • How are the genes delivered?
  • How should I resuspend my genes?
  • I got my genes, but the yield says “NA”. What does that mean?
  • How is processing different when my gene is delivered in a plate versus having it delivered in a tube?
  • How much of my DNA is delivered?
  • Do I need to include restriction sites or overhangs on my sequences?
  • What is the Turn Around Time (TAT) for gene synthesis?
  • How do I find out the arrangement of my genes in your 96-well plate?
  • What is the minimum size gene I can purchase?
  • What is the maximum size gene I can purchase?
  • What is the difference between Twist's Clonal Genes and Gene Fragments?

Gene Fragments

  • What is the price for a gene fragment?
  • What methods can I use to clone my adapter-on gene fragments?
  • Are adapter sequences appended onto the ends of my sequences?
  • Are Gene Fragments purified?
  • What are the adapter sequences used on the Adapter-On Gene Fragments?

Clonal Genes

  • Can I get a glycerol stock of my clonal gene?
  • What is the price for a clonal gene?
  • Can Twist provide cloned products?
  • Does Twist offer plant expression vectors?
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