General

Twist’s Multiplexed Gene Fragments (MGF) offer a unique pooled format for up to hundreds of thousands of double-stranded DNA gene fragments, between 301 and 500 base pairs in length. This pooled format of fragments enables high-throughput screening applications such as prime editing, ultra-complex CRISPR-based functional screening, peptide and protein engineering, Ab discovery, and massively parallel reporter assays (MPRA), where cost per fragment can be a barrier to the desired number of variants.

Twist uses phosphoramidite chemistry for synthesis. Compared to conventional column-based synthesizers, our synthesis platform miniaturizes the synthesis process, which reduces reaction volumes and increases throughput. This process has been optimized over the years and now allows Twist to print pools that are up to 500 nucleotides in length. After synthesis, we have an optimized amplification workflow to generate the multiplexed gene fragments in a dsDNA format.

Twist uses an optimized amplification workflow to generate the multiplexed gene fragments in a dsDNA format which mitigates the risk of poor amplification results as PCR amplification of the synthesized material can be very challenging.

Twist’s Multiplexed Gene Fragments (MGF) offer a unique pooled format for up to hundreds of thousands of double-stranded DNA gene fragments, between 301 and 500 base pairs in length. This pooled format of fragments enables high-throughput screening applications such as prime editing, ultra-complex CRISPR-based functional screening, peptide and protein engineering, Ab discovery, and massively parallel reporter assays (MPRA), where cost per fragment can be a barrier to the desired number of variants.

Twist uses phosphoramidite chemistry for synthesis. Compared to conventional column-based synthesizers, our synthesis platform miniaturizes the synthesis process, which reduces reaction volumes and increases throughput. This process has been optimized over the years and now allows Twist to print pools that are up to 500 nucleotides in length. After synthesis, we have an optimized amplification workflow to generate the multiplexed gene fragments in a dsDNA format.

Twist uses an optimized amplification workflow to generate the multiplexed gene fragments in a dsDNA format which mitigates the risk of poor amplification results as PCR amplification of the synthesized material can be very challenging.