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  • What method of fragmentation can I use in this workflow?
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  • Can I multiplex libraries in the capture; if so, what is the amount of library required?
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  • What are the recommended number of amplification cycles post-capture?
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What are the recommended number of amplification cycles post-capture?

See table: 

Panel Size Custom Panel Custom Methylation Panel
>100 Mb 5 8
50-100 Mb 7 9
25-50 Mb 8 10
10-25 Mb 8 11
2.5-10 Mb 9 12
1-2.5 Mb 9 13
500-1,000 kb 11 14
100-500 kb 13 15
50-100 kb 14 16
<50 kb 15 17

 

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