What is the min/max of DNA input for library preparation?
We recommend a minimum of 10-20 ng of high-quality DNA and maximum of 200 ng.
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Methylation
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- What method of fragmentation can I use in this workflow?
- What is the recommended insert size?
- What is the final library size?
- What is my expected final library yield?
- Are there any internal controls to test for methylation conversion?
- What is the min/max of DNA input for library preparation?
- Can I use the Standard Hybridization workflow for enrichment?
- Can I multiplex libraries in the capture; if so, what is the amount of library required?
- What modifications to the protocol can be made to optimize hybrid capture?
- What are the recommended number of amplification cycles post-capture?
- Should I use the Methylation Enhancer?
