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Consistent performance across inputs and targets
EM-seq v2 Methylation Detection System is an enzymatic library preparation kit that preserves DNA methylation signatures for sequencing, enabling accurate CpG methylation detection while delivering consistent library yields across a wide range of sample inputs.
Preserve your sample
Traditional bisulfite workflows damage DNA. EM-seq v2 uses enzymatic conversion to maintain integrity.
Get reliable methylation calls
High conversion efficiency ensures accurate CpG detection across targets.
Work with less input
Generate consistent libraries from as little as 0.1 ng DNA with reduced dropout.
Product data
Libraries prepared with EM-Seq v2 maintain capture efficiency and uniformity. Performance is preserved through downstream workflows specifically for methylation applications, including compatibility with both standard and fast hybridization protocols.
Figure 1. The Twist Methylation Enhancer improves target enrichment quality and reduces methylation-specific off-target hybridization for Twist EM-Seq v2 libraries captured with the Twist Alliance Pan-Cancer panel, using either the Fast or Standard Hybridization v2 systems.
EM-Seq v2 libraries processed with Twist hybridization maintain consistent capture efficiency and methylation performance, supporting reliable downstream analysis.
Figure 2. Twist EM-Seq v2 Methylation Detection System libraries captured with the Twist Alliance Pan-Caner panel using the Twist Standard Hybridization v2 system provide highly reproducible Picard target enrichment performance metrics across samples with highly variable methylation levels.
Consistent detection of differentially methylated regions demonstrates reliable performance across methylation targets, reinforcing reproducibility for downstream methylation analysis.
Figure 3. Highly sensitive methylation detection. Detection of methylation is possible across a wide range of methylation levels and targets.
EM-Seq v2 generates consistent, usable library yields across a wide DNA input range. Reduced dropout at lower inputs supports methylation sequencing when sample availability is limited, while maintaining reliable performance across workflows.
Figure 4. Yield of libraries generated with Twist EM-seq v2 Methylation Detection System. Library yields are high even with low mass input.
EM-Seq v2 delivers robust enzymatic conversion of unmethylated cytosines with consistent CpG call ratios, supporting accurate and reliable methylation analysis while preserving DNA integrity.
Figure 5. Left Panel: Non-CPG Conversion Rate. Conversion rates with v2 are high even with low mass input. Right Panel: Called CPG Ratio. Conversion specificity with v2 is high even with low mass input.
Video
Engineered reagents preserve performance post-conversion
Libraries prepared with EM-Seq v2 maintain capture efficiency and uniformity. Performance is preserved through downstream workflows specifically for methylation applications, including compatibility with both standard and fast hybridization protocols.
Figure 1. The Twist Methylation Enhancer improves target enrichment quality and reduces methylation-specific off-target hybridization for Twist EM-Seq v2 libraries captured with the Twist Alliance Pan-Cancer panel, using either the Fast or Standard Hybridization v2 systems.
Consistent methylation hybridization capture performance
EM-Seq v2 libraries processed with Twist hybridization maintain consistent capture efficiency and methylation performance, supporting reliable downstream analysis.
Figure 2. Twist EM-Seq v2 Methylation Detection System libraries captured with the Twist Alliance Pan-Caner panel using the Twist Standard Hybridization v2 system provide highly reproducible Picard target enrichment performance metrics across samples with highly variable methylation levels.
Reproducible methylation profiling across targets
Consistent detection of differentially methylated regions demonstrates reliable performance across methylation targets, reinforcing reproducibility for downstream methylation analysis.
Figure 3. Highly sensitive methylation detection. Detection of methylation is possible across a wide range of methylation levels and targets.
Consistent library yield across DNA inputs
EM-Seq v2 generates consistent, usable library yields across a wide DNA input range. Reduced dropout at lower inputs supports methylation sequencing when sample availability is limited, while maintaining reliable performance across workflows.
Figure 4. Yield of libraries generated with Twist EM-seq v2 Methylation Detection System. Library yields are high even with low mass input.
Confident enzymatic conversion for accurate CpG methylation calling
EM-Seq v2 delivers robust enzymatic conversion of unmethylated cytosines with consistent CpG call ratios, supporting accurate and reliable methylation analysis while preserving DNA integrity.
Figure 5. Left Panel: Non-CPG Conversion Rate. Conversion rates with v2 are high even with low mass input. Right Panel: Called CPG Ratio. Conversion specificity with v2 is high even with low mass input.
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Engineered reagents preserve performance post-conversion
Libraries prepared with EM-Seq v2 maintain capture efficiency and uniformity. Performance is preserved through downstream workflows specifically for methylation applications, including compatibility with both standard and fast hybridization protocols.
Consistent methylation hybridization capture performance
EM-Seq v2 libraries processed with Twist hybridization maintain consistent capture efficiency and methylation performance, supporting reliable downstream analysis.
Reproducible methylation profiling across targets
Consistent detection of differentially methylated regions demonstrates reliable performance across methylation targets, reinforcing reproducibility for downstream methylation analysis.
Consistent library yield across DNA inputs
EM-Seq v2 generates consistent, usable library yields across a wide DNA input range. Reduced dropout at lower inputs supports methylation sequencing when sample availability is limited, while maintaining reliable performance across workflows.
Confident enzymatic conversion for accurate CpG methylation calling
EM-Seq v2 delivers robust enzymatic conversion of unmethylated cytosines with consistent CpG call ratios, supporting accurate and reliable methylation analysis while preserving DNA integrity.
For Research Use Only. Not for use in diagnostic procedures.
NEBNext® and EM-seq™ are registered trademarks or trademarks of New England Biolabs, Inc. Used with permission or under license where applicable.
