Twist Mechanical Fragmentation for PCR Free Whole Genome Sequencing

This Twist Whole Genome Sequencing Workflow details all the necessary steps for generating PCR-free indexed libraries for sequencing on Illumina next-generation sequencing (NGS) systems. The workflow prepares genomic DNA (gDNA) libraries using mechanically fragmented DNA and full-length Y-shaped adapters. This library preparation workflow has been optimized for use with the reagents specified and should only be performed with them or their equivalents.

Covered in this Protocol

PCR-Free Whole Genome Sequencing Workflow with Mechanical Fragmentation

Materials Supplied by User

General Notes and Precautions

Guidelines for Samples

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Twist Mechanical Fragmentation for PCR Free Whole Genome Sequencing

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