Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers.
Twist Universal Adapters
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist 10 bp Unique Dual-Indexed (UDI) Primers
Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
Twist 12bp High Throughput (HT) UDI Primers
Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.
Twist’s Universal Adapter Systems improve the performance of any ‘T-A’ overhang ligation library preparation workflow for next-generation sequencing (NGS) applications. Engineered to optimize the performance of Twist’s Library Preparation and Target Enrichment products, the systems consist of Twist’s Universal Adapters and either Twist Unique Dual-Indexed (UDI) Primers or Twist HT UDI Primers.
Twist Universal Adapters
The proprietary design of the universal TruSeq-compatible adapters ensures compatibility with all ‘T-A’ overhang workflows and eliminates formation of undesired adapter dimer. It also contributes to increasing the workflow’s overall yield, which is especially beneficial prior to a target enrichment step.
Twist 10 bp Unique Dual-Indexed (UDI) Primers
Twist’s UDI primers contain mixed primer pairs, which enable higher confidence sample pooling than combinatorial approaches. The index design allows for correction of substitutions, insertions, and deletions within the index reads during post-sequencing processing.
Twist 12bp High Throughput (HT) UDI Primers
Available in automation-friendly 96-well or 384-well plate formats, Twist’s HT UDI primers offer the most scalable solution for high throughput sequencing. All 3,072 unique indexes take into consideration base and color channel balancing, making them optimal for either two-color or four-color chemistries.
Looking for a streamlined method to detect sample mix-ups?
See how the Twist Human Sample ID kit integrates with Twist’s Universal Adapter Systems.
Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”
The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.
Library preparation and target enrichment work together to influence the ability to call variants in sequencing. Consistently constructing robust libraries is important for successful hybrid capture. When adapters dimerize during library preparation, they generate undesired products and reduce the effective concentration of adapters available for ligation to inserts. Using Twist Universal Adapters and Primers during library construction minimizes this problem.
Twist Universal Adapters minimize adapter dimer formation during library preparation.
Twist Universal Adapters and HT Universal Adapters return higher total yields with fewer PCR cycles. This ultimately leads to the production of more library with fewer PCR errors to drive improvements in target enrichment efficiency.”
The chart to the right shows how using Twist Universal Adapters for insert ligation and UDI primer PCR amplification generates a robust library.
Library preparation and target enrichment work together to influence the ability to call variants in sequencing. Consistently constructing robust libraries is important for successful hybrid capture. When adapters dimerize during library preparation, they generate undesired products and reduce the effective concentration of adapters available for ligation to inserts. Using Twist Universal Adapters and Primers during library construction minimizes this problem.
Twist Universal Adapters minimize adapter dimer formation during library preparation.
101307
Twist Universal Adapter System TruSeq Compatible, 16 Samples101308
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate A101309
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate B101310
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate C101311
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate D106390
Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04106391
Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08106392
Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12106393
Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16106396
Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)106398
Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)106399
Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)106400
Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)106401
Twist HT Universal Adapter System TruSeq Comp, 384W AAA01106402
Twist HT Universal Adapter System TruSeq Comp, 384W AAA02106403
Twist HT Universal Adapter System TruSeq Comp, 384W AAA03106404
Twist HT Universal Adapter System TruSeq Comp, 384W AAA04106405
Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)106406
Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)106407
Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)106408
Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)The Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
101307
Twist Universal Adapter System TruSeq Compatible, 16 Samples101308
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate A101309
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate B101310
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate C101311
Twist Universal Adapter System TruSeq Compatible, 96 Samples Plate D106390
Twist HT Universal Adapter System TruSeq Comp, 96W AAA01-04106391
Twist HT Universal Adapter System TruSeq Comp, 96W AAA05-08106392
Twist HT Universal Adapter System TruSeq Comp, 96W AAA09-12106393
Twist HT Universal Adapter System TruSeq Comp, 96W AAA13-16106396
Twist HT Universal Adapter System TruSeq Comp, 96W BBB01-04 (Coming soon!)106398
Twist HT Universal Adapter System TruSeq Comp, 96W BBB05-08 (Coming soon!)106399
Twist HT Universal Adapter System TruSeq Comp, 96W BBB09-12 (Coming soon!)106400
Twist HT Universal Adapter System TruSeq Comp, 96W BBB13-16 (Coming soon!)106401
Twist HT Universal Adapter System TruSeq Comp, 384W AAA01106402
Twist HT Universal Adapter System TruSeq Comp, 384W AAA02106403
Twist HT Universal Adapter System TruSeq Comp, 384W AAA03106404
Twist HT Universal Adapter System TruSeq Comp, 384W AAA04106405
Twist HT Universal Adapter System TruSeq Comp, 384W BBB01 (Coming soon!)106406
Twist HT Universal Adapter System TruSeq Comp, 384W BBB02 (Coming soon!)106407
Twist HT Universal Adapter System TruSeq Comp, 384W BBB03 (Coming soon!)106408
Twist HT Universal Adapter System TruSeq Comp, 384W BBB04 (Coming soon!)The Twist Universal Adapter System is for research use only, and subject to additional use restrictions as set forth in Twist’s Supply Terms and Conditions.
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible (Plate A), 96 Samples (SDS-001088)
Safety Data Sheet for 101308
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible (Plates B, C, and D), 96 Samples (SDS-001089)
Safety Data Sheet for 101309 - 101311
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible, 16 Samples (SDS-001122)
Safety Data Sheet for 101307
Safety Data Sheet
Twist Universal Adapters - TruSeq Comp, 384 Samples (SDS-001125)
Safety Data Sheet for 104439
Safety Data Sheet
Twist HT UDI Primers - TruSeq Comp, Plate 96 Well Plates (SDS-001124)
Safety Data Sheets for 104493 - 10452
Safety Data Sheet
Twist HT UDI Primers - TruSeq Comp, 384 Well Plates (SDS-001123)
Safety Data Sheet for 104431-104438
Poster
UDI Primers and Universal Blockers Enable Multiplexed Target Enrichments (ASHG 2019)
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible (Plate A), 96 Samples (SDS-001088)
Safety Data Sheet for 101308
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible (Plates B, C, and D), 96 Samples (SDS-001089)
Safety Data Sheet for 101309 - 101311
Safety Data Sheet
Twist Universal Adapter System - TruSeq Compatible, 16 Samples (SDS-001122)
Safety Data Sheet for 101307
Safety Data Sheet
Twist Universal Adapters - TruSeq Comp, 384 Samples (SDS-001125)
Safety Data Sheet for 104439
Safety Data Sheet
Twist HT UDI Primers - TruSeq Comp, Plate 96 Well Plates (SDS-001124)
Safety Data Sheets for 104493 - 10452
Safety Data Sheet
Twist HT UDI Primers - TruSeq Comp, 384 Well Plates (SDS-001123)
Safety Data Sheet for 104431-104438
Poster
UDI Primers and Universal Blockers Enable Multiplexed Target Enrichments (ASHG 2019)