Public health efforts to track and stem HIV transmission are hindered by viral latency, treatment delay between infection and clinical diagnosis, and for some people, lapses in care that obscure data sets. Wastewater can reveal a broad, immediate, and unbiased accounting of the health of the served population and was historically used to monitor pathogens of pandemic potential. We aimed to track HIV in wastewater utilizing a viral detection pipeline adapted from platforms developed to track SARS-COV-2. We validated published PCR primers with wastewater nucleic acid extracts and found the gag gene primers most reliable for detection. Interestingly, wastewater from regions of higher and lower HIV prevalence both produced detections by RT-PCR. Sequencing of both RT-PCR products and nucleic acid extracts was consistent with HIV detection. This work provides the first evidence HIV can be reliably and consistently detected in municipal wastewater systems and develops methods and a pipeline for HIV detection.