Gene Fragments

For standard complexity sequences and considering our error rate of 1 in 7500, selecting the recommended number of colonies, as shown in the graph, provides a 95% probability of finding a colony with the correct sequence.

Please note that this recommendation is a starting point. Various factors, such as vector type, sequence complexity, and sequence content, can affect colony growth. In some scenarios, selecting 2 to 3 times the recommended number of colonies may be necessary.

You can find the pricing table for Gene Fragment products in your account by clicking here.

If you need to contact your Account Manager regarding pricing, their contact information is available in eCommerce under Account.

Certificates of Analysis (COA) are available for completed orders. You can find the COA for Gene Fragment products in your account by clicking here.

• For orders received before August 9, 2021, the adapter sequences are as follows:
  • 5' Adapter: GAAGTGCCATTCCGCCTGACCT
  • 3' Adapter: AGGCTAGGTGGAGGCTCAGTG
  • 5’ – GAAGTGCCATTCCGCCTGACCT – insert – AGGCTAGGTGGAGGCTCAGTG – 3'

• For orders received after August 9, 2021 to November 1, 2021, the adapter sequences are as follows:
  • 5' Adapter: CCCCGTCACCTTTGGCTTATCAGT
  • 3' Adapter: AGTGACATCTGGACGCTAAGACCG
  • 5’ - CCCCGTCACCTTTGGCTTATCAGT – insert – AGTGACATCTGGACGCTAAGACCG - 3'

• For orders received after November 2, 2021, the adapter sequences are as follows:
  • 5' Adapter: CAATCCGCCCTCACTACAACCG
  • 3' Adapter: CTACTCTGGCGTCGATGAGGGA
  • 5’ - CAATCCGCCCTCACTACAACCG – insert – CTACTCTGGCGTCGATGAGGGA - 3'

*Adapters are short sequences added to each end of the insert as part of our synthesis process. Gene fragments without these adapters are also available during the checkout process.

If you have any questions about your order, please contact us at customersupport@twistbioscience.com

For Adapter-On Gene Fragments, universal adapters are synthesized onto the ends of each fragment. The adapters are 21-22 nucleotides in length. Fragments with these adapters are compatible with a number of assembly methods, namely Restriction Digestion/Ligation, Gateway, Golden Gate, and TOPO cloning.

The addition of restriction sites inside of the adapters can be used to remove adapters. TOPO cloning will work but will leave the adapters on. 

ADAPTER SEQUENCES

• For orders received before August 9, 2021, the adapter sequences are as follows:
  • 5' Adapter: GAAGTGCCATTCCGCCTGACCT
  • 3' Adapter: AGGCTAGGTGGAGGCTCAGTG
  • 5’ – GAAGTGCCATTCCGCCTGACCT – insert – AGGCTAGGTGGAGGCTCAGTG – 3'

• For orders received after August 9, 2021 to November 1, 2021, the adapter sequences are as follows:
  • 5' Adapter: CCCCGTCACCTTTGGCTTATCAGT
  • 3' Adapter: AGTGACATCTGGACGCTAAGACCG
  • 5’ - CCCCGTCACCTTTGGCTTATCAGT – insert – AGTGACATCTGGACGCTAAGACCG - 3'

• For orders received after November 2, 2021, the adapter sequences are as follows:
  • 5' Adapter: CAATCCGCCCTCACTACAACCG
  • 3' Adapter: CTACTCTGGCGTCGATGAGGGA
  • 5’ - CAATCCGCCCTCACTACAACCG – insert – CTACTCTGGCGTCGATGAGGGA - 3'

*Adapters are short sequences added to each end of the insert as part of our synthesis process. Gene fragments without these adapters are also available during the checkout process. 

If you have any questions about your order, please contact us at customersupport@twistbioscience.com  

You can use the following methods to clone gene fragments with adapters:

Restriction digestion/Ligation - place restriction sites inside of the Twist adapters for easy cloning

Type IIS or Golden Gate - place restriction sites inside of the Twist adapters for easy cloning TOPO cloning (blunt-end) - the adapter sequences will be cloned in as well

Gateway cloning - add attB sites to the ends of your sequence

For more information, click here.  

Yes, gene fragments go through a bead-based clean up; however, short fragments can carry over in small quantities.

For standard complexity sequences and considering our error rate of 1 in 7500, selecting the recommended number of colonies, as shown in the graph, provides a 95% probability of finding a colony with the correct sequence.

Please note that this recommendation is a starting point. Various factors, such as vector type, sequence complexity, and sequence content, can affect colony growth. In some scenarios, selecting 2 to 3 times the recommended number of colonies may be necessary.

You can find the pricing table for Gene Fragment products in your account by clicking here.

If you need to contact your Account Manager regarding pricing, their contact information is available in eCommerce under Account.

Certificates of Analysis (COA) are available for completed orders. You can find the COA for Gene Fragment products in your account by clicking here.

• For orders received before August 9, 2021, the adapter sequences are as follows:
  • 5' Adapter: GAAGTGCCATTCCGCCTGACCT
  • 3' Adapter: AGGCTAGGTGGAGGCTCAGTG
  • 5’ – GAAGTGCCATTCCGCCTGACCT – insert – AGGCTAGGTGGAGGCTCAGTG – 3'

• For orders received after August 9, 2021 to November 1, 2021, the adapter sequences are as follows:
  • 5' Adapter: CCCCGTCACCTTTGGCTTATCAGT
  • 3' Adapter: AGTGACATCTGGACGCTAAGACCG
  • 5’ - CCCCGTCACCTTTGGCTTATCAGT – insert – AGTGACATCTGGACGCTAAGACCG - 3'

• For orders received after November 2, 2021, the adapter sequences are as follows:
  • 5' Adapter: CAATCCGCCCTCACTACAACCG
  • 3' Adapter: CTACTCTGGCGTCGATGAGGGA
  • 5’ - CAATCCGCCCTCACTACAACCG – insert – CTACTCTGGCGTCGATGAGGGA - 3'

*Adapters are short sequences added to each end of the insert as part of our synthesis process. Gene fragments without these adapters are also available during the checkout process.

If you have any questions about your order, please contact us at customersupport@twistbioscience.com

For Adapter-On Gene Fragments, universal adapters are synthesized onto the ends of each fragment. The adapters are 21-22 nucleotides in length. Fragments with these adapters are compatible with a number of assembly methods, namely Restriction Digestion/Ligation, Gateway, Golden Gate, and TOPO cloning.

The addition of restriction sites inside of the adapters can be used to remove adapters. TOPO cloning will work but will leave the adapters on. 

ADAPTER SEQUENCES

• For orders received before August 9, 2021, the adapter sequences are as follows:
  • 5' Adapter: GAAGTGCCATTCCGCCTGACCT
  • 3' Adapter: AGGCTAGGTGGAGGCTCAGTG
  • 5’ – GAAGTGCCATTCCGCCTGACCT – insert – AGGCTAGGTGGAGGCTCAGTG – 3'

• For orders received after August 9, 2021 to November 1, 2021, the adapter sequences are as follows:
  • 5' Adapter: CCCCGTCACCTTTGGCTTATCAGT
  • 3' Adapter: AGTGACATCTGGACGCTAAGACCG
  • 5’ - CCCCGTCACCTTTGGCTTATCAGT – insert – AGTGACATCTGGACGCTAAGACCG - 3'

• For orders received after November 2, 2021, the adapter sequences are as follows:
  • 5' Adapter: CAATCCGCCCTCACTACAACCG
  • 3' Adapter: CTACTCTGGCGTCGATGAGGGA
  • 5’ - CAATCCGCCCTCACTACAACCG – insert – CTACTCTGGCGTCGATGAGGGA - 3'

*Adapters are short sequences added to each end of the insert as part of our synthesis process. Gene fragments without these adapters are also available during the checkout process. 

If you have any questions about your order, please contact us at customersupport@twistbioscience.com  

You can use the following methods to clone gene fragments with adapters:

Restriction digestion/Ligation - place restriction sites inside of the Twist adapters for easy cloning

Type IIS or Golden Gate - place restriction sites inside of the Twist adapters for easy cloning TOPO cloning (blunt-end) - the adapter sequences will be cloned in as well

Gateway cloning - add attB sites to the ends of your sequence

For more information, click here.  

Yes, gene fragments go through a bead-based clean up; however, short fragments can carry over in small quantities.