What methods can I use to clone my adapter-on gene fragments?
You can use the following methods to clone gene fragments with adapters:
Restriction digestion/Ligation - place restriction sites inside of the Twist adapters for easy cloning
Type IIS or Golden Gate - place restriction sites inside of the Twist adapters for easy cloning TOPO cloning (blunt-end) - the adapter sequences will be cloned in as well
Gateway cloning - add attB sites to the ends of your sequence
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