Introducing Complex Gene Synthesis We make challenging DNA simple. Starting at 12¢ per base.

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Gene Synthesis

Complex DNA.
Unlimited scale.
One promise.

You never need to compromise on gene synthesis again.
We deliver affordable, reliable, quality DNA fast. Every time.

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Affordable pricing at every level of complexity

Get the most from your budget with Gene Fragments from 7¢ per base, Clonal Genes from 9¢ per base, and Complex Clonal Genes from 12¢ per base.
Reliable synthesis with predictable turnaround

Get your DNA when you expect it. Gene Fragments are shipped in as fast as 2 days. Clonal Genes are shipped in as fast as 4 days.
Simple, customizable ordering

Stay on top of every order with your Twist account. Optimize sequences for expression, customize delivery formats, and get instant quotes.
Complete transparency from order to delivery

Keep to plan with detailed order tracking. Get realistic manufacturability data before you order. Get status updates in real time as your DNA is made.

Gene synthesis from

$0.07/bp

Delivered as fast as

2 BD^*

With an acceptance rate of

>99.5%

Clonal Genes

For researchers that need reliable, sequence-perfect DNA they can drop into downstream workflows

Length: 0.3 kb – 7.0 kb

Scale: 1 µg – 4 µg | 4 µg – 10 µg | 10 µg – 100 µg | 100 µg – 1 mg^†

Price (by complexity level):
0.3 – 1.8 kb: 9¢/bp (Low/Medium) | 12¢/bp (High)^‡
1.8 – 3.2 kb: 13¢/bp (Low/Medium) | 18¢/bp (High)
3.2 – 7.0 kb: 18¢/bp (Low/Medium) | 23¢/bp (High)
Cloning: Cloning vector: $35 | Expression vector: $55 | Custom vector: $75

Turnaround time (in business days): Express: 4 – 7 | Standard: 10 | High complexity: 15

Customization options: Animal-Origin-Free prep, BSE/TSE certification, Transfection grade preps, Endotoxin free, Target yield, Poly-A QC

Error rate: 100% sequence perfect, NGS verified

^†For high copy vectors. Low/Medium copy vector performance will vary.
^‡0.3 – 0.5 kb genes have a per-sequence price which includes cloning and synthesis.

Order Clonal Genes See the data

Gene Fragments

For researchers that need affordable DNA delivered as fast as possible

Length: 0.3 kb – 5.0 kb

Scale: 100 ng – 1 µg

Price:
0.3 – 0.5 kb: $35/sequence
0.5 – 1.8 kb: 7¢/bp
1.8 – 3.2 kb: 8¢/bp
3.2 – 5.0 kb: 9¢/bp

Turnaround time (in business days): 2 – 4

Error rate: 1:7500

Order Gene Fragments See the data

Need genes for high-throughput screens?

Multiplexed Gene Fragments Like long oligos, but even better Pooled dsDNA sourced directly from the writer, available up to 500bp. Learn more Gene Pools The longest pooled DNA product on the market Chimera-free pooled gene fragments, assembled up to 1.8kb. Learn more

Drag and drop files or paste sequences directly into the table. Our interface supports bulk management and formatted templates to get your data structured and ready in seconds.

Choose from over 60 vectors in our extensive catalog, or onboard your own vector for us to clone into.

Our scoring algorithm provides a score and granular detail, highlighting complexities and their locations.

Have the option to codon optimize your sequences in bulk, or one by one with over 150 host organism species to select from.

Add a custom plate map, or choose scale, grade, and documentation you wish to receive.

Add payment methods, shipping addresses, get a detailed quote and once your order is placed, track the status of each of the items in the order, or download the documentation for specific orders.

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Integrate our quick-check tool into any DNA-design workflow, and never submit a rejected sequence again. Spot errors or complexities, and know in seconds which synthesis solutions are optimal for your designs.

Check your sequences

Unlock the full potential of your DNA in seconds with our state-of-the-art codon optimization tool. Our AI-driven algorithm is built to maximize downstream protein expression without compromising manufacturability.

Start optimizing

Skip molecular biology with our ready-to-use vectors available immediately in your Twist account. Explore detailed vector maps and find the perfect backbone for your project.

Explore vectors

Manufacturability analyzer

QC your sequence designs

Integrate our quick-check tool into any DNA-design workflow, and never submit a rejected sequence again. Spot errors or complexities, and know in seconds which synthesis solutions are optimal for your designs.

Check your sequences

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*For Research Use Only. Not for Diagnostic Procedures.

Genes in action

Success stories and studies featuring Twist Genes

Compressing plant trait discovery timelines by 90% to generate more disease resilient crops

1 year

Plant trait engineering timeline

10,000s

Of plant effectors screened

Discover how massive-scale synthesis is being paired with AI-driven trait engineering to circumvent the evolutionary “arms race”, and generate crops protected from disease.

Products used

Gene Fragments

Read case study

Compressing plant trait discovery timelines by 90% to generate more disease resilient crops

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“A key part of our work with external partners is keeping to our agreed project delivery timelines. Having a reliable synthesis partner in Twist is a huge factor in the success of this work, as they make it easy to track the progress of my order, deliver genes on time, and actively communicate whenever there is an issue.”

Imogen Binnan, PhD

Lead Biologist, Resurrect Bio

*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions

“The online ordering experience on Twist is by far the smoothest and most delightful of all providers I have tried. Together with the competitive pricing, Twist is our team’s go-to supplier for DNA Fragments, Clonal Genes, and Oligo Pools.”

David Lips

Frontier Development, Spiber Inc.

*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions

“I am a structural biologist working on proteins with diverse functions from a wide range of organisms. Our research requires extensive gene screening, and therefore gene synthesis must be accurate, fast, and cost-effective. Genes synthesized by Twist Bioscience have consistently shown a low error rate and reliable quality, while being delivered rapidly and at a reasonable cost. We look forward to continuing to work with Twist Bioscience in the future.”

Masahiro Fukuda, Ph.D.

Assistant Professor, The Research Center for Advanced Science and Technology (RCAST) The University of Tokyo

*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions

“Twist Bioscience saved me during my PhD! I ordered countless synthetic genes from them. Whenever a cloning procedure didn’t work in lab, they provided the entire plasmid, which made a huge difference. I even used their services to build our own nanobody library. Their reliable and high-quality products were essential to the success of my research!!”

Claudia D’Ercole

Postdoctoral researcher, Laboratory for Environmental and Life Sciences, Univerza v Novi Gorici, Slovenija

*Results are specific to the institution where they were obtained and may not reflect the results achievable at other institutions

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Antibody Discovery Services

Partner with our team of experts to navigate the entire discovery funnel from target to lead.

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Oligo Pools

High-diversity, silicon-synthesized oligo pools designed for high-throughput screening and complex library construction.

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Libraries

Explore vast sequence space with high-diversity libraries where every variant is base-by-base synthesized and NGS-verified to match your design.

Explore

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Complex DNA.
Unlimited scale.
One promise.

Keep your projects on track with Twist Gene Synthesis

Affordable pricing at every level of complexity

Reliable synthesis with predictable turnaround

Simple, customizable ordering

Complete transparency from order to delivery