Oligo Pools for High Throughput Screens

Products

Applications

Resources

COMPANY

OVERVIEW
OLIGO POOLS
CLONED OLIGO POOLS
APPLICATIONS
RESOURCES

ORDER NOW

Performance at any scale

Our revolutionary silicon-based DNA Synthesis platform is capable of producing over a million unique ssDNA oligos in a single run. Each chip contains thousands of discrete clusters each containing 121 individually addressable surfaces capable of synthesizing a unique oligo sequence. A representation of the silicon chip technology can be seen below.

Due to the sheer scale of DNA synthesis capability on the chip there are virtually no limitations to the number of unique oligos that can be assembled into a pool. Don't restrict the outcome of your application, order Oligo Pools tailored precisely to your experiment.

More Space for Innovation

Don’t be limited by length. Encode the elements and sequences you need, including promoters, enhancers, guides, and probes all within the expanded architecture. With oligo lengths up to 300nt it’s possible to encode multiple elements in a single design giving you the ability to do more with each oligo.

Screen once, screen right

Unmatched pool uniformity without bias provides more unique targets per screen. This means less effort is required to achieve the data coverage you need, saving time and money.

Be data confident

With the precision and uniformity of Twist Oligo Pools, you can be confident in the quality of your experimental results. Don’t miss a datapoint due to synthesis errors or inefficiencies. Get industry-leading oligo representation and low error rate for oligos of any length up to 300nt, and pools of any size. Leverage these exceptional quality oligo pools at the precise scale and length required for your application.

HAVE QUESTIONS?

Tell us what you would like more information on. We’re here to help!

WHY DOES UNIFORMITY MATTER?

Oligo Pools

Twist Oligo Pools are highly diverse collections of single-stranded oligonucleotides synthesized using our silicon-based DNA writing technology. Our synthesis platform enables massively parallel production of hundreds of thousands of high-quality, accurate oligos per run, allowing the generation of complex and diverse oligo pools for use in applications like CRISPR screening.

How does it work?

You provide us with your oligo sequences and we will synthesize your user designed oligo pool libraries, enabling you to spend your time working on experimentation and discovery.

Highly Uniform Oligo Synthesis

NGS quality control data from a typical oligo pool containing 23,000 90mer oligos show the uniformity of the pool at 300x read coverage. The corresponding table indicates the uniformity metrics for this pool. Twist oligos are synthesized bias-free with high uniformity and complete oligo representation.

Sequencing Analysis of Oligo Pools

Sequencing analysis of Oligo Pools generated by Twist Bioscience and an array-based competitor demonstrate that the Twist Oligo Pools contain 100% of the expected sequences and a higher percentage of correct sequences than the competitor pool.

Specifications

Oligo Length	Up to 300nt
Oligo Pool Size	No limits on pool size
Yield	>0.2 fmol average of each oligo
Uniformity	>90% oligos represented within <2.0x of the mean
Error rate	Up to 1:3000
Turn-Around Time	2-4 business days*
Price	Industry-leading pricing

*Twist Internal Data 2025. Time frame of 2-4 business days for Twist Oligo Pools up to 300 nt is based on internal data available as of 2025. This timeframe refers to the typical processing and handling time within our facilities before your order is handed over to the shipping carrier. Actual delivery times will vary depending on your location, the chosen shipping method, and the carrier's handling procedures.

How AI and Twist DNA synthesis are powering high throughput plant trait discovery

Phytoform Labs used oligo pools to screen thousands of AI-designed DNA sequences, streamlining trait engineering and accelerating crop improvement.

Cloned Oligo Pools

Oligo pool quality is the foundation of a successful experiment. Errors during synthesis or cloning can easily skew oligo pool quality, leading to over and underrepresentation of desired sequences. Twist now offers an optimized cloning service that allows you to further avoid the hardship that comes with testing PCR amplification conditions, selecting the right polymerases and primer pairs, as well as designing a cloning workflow. Skip the drama and let Twist Bioscience synthesize and clone oligo pools for you.

How does it work?

Just two steps away from your custom cloned Oligo Pools. You provide us with your oligo sequences and we will synthesize, amplify, and clone your user designed oligo pool libraries, enabling you to spend your time working on experimentation and discovery.

Twist Bioscience’s cloned oligo pools of all lengths have high uniformities and low error rates

Performance data of amplified and cloned oligo pools under 150 nucleotides in length even with high GC content:

Performance data of amplified and cloned oligo pools up to 300 nucleotides in length even with high GC content:

Achieve the highest quality and accuracy in your experimental data

Twist’s cloned oligo pools not only have low error rates and high uniformity, but also have low chimera rates. Chimeras are unwanted hybrid molecules that are created as a result of suboptimal PCR conditions which leads to improper amplification and recombination of different oligos within the oligo pool. See our illustration below for an example of an on-target cloned pool versus an off-target one that contains chimeras, which ultimately leads cloned oligo pools that are comprised of undesired sequences:

With Twist, you never have to worry about Chimeras! See for yourself:

Traditional Amplification vs Twist In-House Amplification Method Performance

Specifications

Oligo Length	Up to 300nt
Oligo Pool Size	No limits on pool size
Yield	50ug of plasmid DNA, option to add up to 250µg and/or glycerol stocks
Uniformity	>90% oligos represented within <5.0x of the mean
Chimera Rate	As low as 1.5%*
Turn-Around Time	As few as 4 weeks*

*Terms and Conditions: Baseline turnaround time for Cloned Oligo Pools, which includes cloning and amplification, of up to 100 and 300 nucleotides is 4-6 weeks and 6-8 weeks, respectively. An additional 2 weeks will be added to this baseline turnaround time for all Cloned Oligo Pools, regardless of length, that require new vector onboarding. Chimera rate, drop out rate, and uniformity will vary based on sequence complexity.

HAVE QUESTIONS?

Tell us what you would like more information on. We’re here to help!

Guide RNA libraries for CRISPR screens

CRISPR screens involve the precise, high-throughput knock-out, interference or modulation of gene activity, allowing complex genotype-phenotype interactions to be unveiled. Oligo Pools are designed to encode multiple guide RNA molecules per gene.

Product benefits include:

Full control over guide library design, avoiding unwanted guides, wasted sequencing, and limited guide-per-gene ratios.
Pairing high-fidelity Cas enzymes with highly accurate synthesis for true high-fidelity screens with minimal off-target effects.
Incredibly uniform, bias-free pools achieve amplified signal to noise ratio to achieve maximum hit detection, lowering downstream costs.
Oligos up to 300nt make space for additional experimental elements. Add multiple guides per oligo, genome homology, or minimal promoters with ease.

Watch our webinar: Scalable and combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted seq

Genome tiling libraries

Tiling libraries allow a comprehensive study of genomic functionality. Pools of oligos may encode peptide arrays, mRNA encoding sequences, or genome fragments.

Product benefits include:

Tiling precise genomic regions of interest. Reduce experimental noise by ignoring unwanted sequences at the design step.
Complex, repetitive or palindromic sequences that cannot typically be synthesized as genes can be encoded in oligos.
Larger peptide structures or genome architectures can be encoded in oligos 300nt in length.
Uniform synthesis minimizes oversampling required for comprehensive screening.

Read our article: Identifying Coronavirus Virulence Factors for RNA Vaccine Development with Twist Oligo Pools

High throughput genetic control screens

Understanding the genetic elements controlling gene expression and regulation has broad application in synthetic biology and development of potential disease treatment. Oligo Pools power high-throughput screens of genetic control, including gene expression profiling and reporter-based applications.

Product benefits include:

High-quality pools ensure you can interrogate every base for functionality
High uniformity means you screen less to find your answers
Up to 300nt pools gives you the flexibility to add multiple elements or homology regions

Watch our webinar: Epitranscriptome Screening on TechNetworks

Fluorescence in-situ hybridization

Fluorescence in-situ hybridization (FISH) allows the abundance and spatial organization of nucleic acids in biological samples to be directly visualized. Oligo Pools can encode probes that when bound to fluorescent markers provide tunable, programmable visualization of large numbers of targets.

Product benefits include:

Rapid FISH probe development
Sequence control enables effective targeting
Longer oligos enable more specific targeting

Watch our webinar: Rapid and efficient production of FISH probes using oligo pools with Nicola Crosetto

Product Sheet

Twist Oligo Pools READ

Twist Cloned Oligo Pools READ

Protocol

Oligo Pools Amplification Guidelines READ

Twist DNA Resuspension Guidelines
These guidelines outline the best practices for resuspending Twist Gene Fragments, Clonal Genes, Oligo Pools, and Variant Libraries. READ

Case Study

Designing Proteins to Revolutionize Biotechnology READ

Webinar

Next generation CRISPR screens based on single-cell transcriptomics WATCH

Using amino acid composition and conservation for more efficient CRISPR screens WATCH

Rapid and efficient production of FISH probes using oligo pools WATCH

Epitranscriptome Screening WATCH

CRISPR CAS9 Screening in Human Pluripotent Stem Cells WATCH

Discovering PARP inhibitor resistance mechanisms using genome-wide and focused CRISPR screens WATCH

What Scientists Have to Say

Twist’s ssDNA oligo pools are well suited for making pooled RNA structures because of their high uniformity, low error rate, and lengths up to 300 nt. These features provide the screening of RNA-small molecule interactions with high accuracy and efficiency..

Kaoru R. Komatsu

Representative Director and Chief Technology Officer • xFOREST Therapeutics Co., Ltd

We have found Twist Oligo Pools to be a fast, convenient and flexible route to synthesizing high quality customized CRISPR libraries for our research..

Stephen Pettit

Staff Scientist • Institute of Cancer Research, UK

We work on massively parallel reporter assays (MPRAs) focused on identifying gene regulatory functions of single base substitutions, so high oligo fidelity is critical. When we sequenced the pool that we got from Twist the quality was astounding, with twofold fewer mutations than we were expecting.

Bernie Mulvey

MD-PhD Student • Washington University School of Medicine • St. Louis, MO, USA

What Scientists Have to Say

“

Kaoru R. Komatsu

Representative Director and Chief Technology Officer • xFOREST Therapeutics Co., Ltd

“

We have found Twist Oligo Pools to be a fast, convenient and flexible route to synthesizing high quality customized CRISPR libraries for our research.

Stephen Pettit

Staff Scientist • Institute of Cancer Research, UK

“

Bernie Mulvey

MD-PhD Student • Washington University School of Medicine • St. Louis, MO, USA

Finally, an easy way to order DNA

0:02

Instantly know if your sequence is a go
Our smart algorithm lets you know in seconds if your sequence can be synthesized or not

Your quote is now just one click away
No more wasted time waiting for surprise quotes. See real-time pricing when you submit your data

Stay in the know from checkout to your door
We update you at every stage of your order’s progress, giving you comprehensive insight on crucial production details

Intuitive usability meets bulletproof security
Upload your sequences with the comfort of knowing your proprietary data is protected

Genes

Oligo Pools

NGS Target Enrichment Solutions

Variant Libraries

Synthetic Controls

Antibody Discovery

Oligo Pools

Performance at any scale

More Space for Innovation

Screen once, screen right

Be data confident

Oligo Pools

How does it work?

Highly Uniform Oligo Synthesis

Sequencing Analysis of Oligo Pools

Specifications

Cloned Oligo Pools

How does it work?

Twist Bioscience’s cloned oligo pools of all lengths have high uniformities and low error rates

Performance data of amplified and cloned oligo pools under 150 nucleotides in length even with high GC content:

Performance data of amplified and cloned oligo pools up to 300 nucleotides in length even with high GC content:

Achieve the highest quality and accuracy in your experimental data

Specifications

​​​​​​Guide RNA libraries for CRISPR screens

Genome tiling libraries

High throughput genetic control screens

Fluorescence in-situ hybridization

What Scientists Have to Say

What Scientists Have to Say

Finally, an easy way to order DNA

Guide RNA libraries for CRISPR screens