Express Service

This indicates that we have reached maximum capacity for the current Express Genes run. Express Genes are run multiple times a day, please check back soon.

Ordering Clonal Genes with our Express service ensures that an order receives top priority at every production stage. With additional resources dedicated to our new tier of service, this prioritized approach allows us to expedite the production of Clonal Genes without compromising quality.

Yes, a fee is added to each gene with the Express service applied. The fee will not be invoiced for any Express Gene that does not ship within the advertised turnaround time.

Express Genes are Clonal Genes produced faster using Twist’s Express service. The Express service reduces production time as follows without compromising quality at any stage.*

1 µg - 4 µg & 4 µg - 10 µg DNA Scale:

Standard: 10 business days

Express: 4-7 business days  

10 µg - 100 µg & 100 µg - 1mg DNA Scale:

Standard: 13 business days

Express: 7-10 business days

*Terms and Conditions: Advertised turnaround time for Express Clonal Genes is 4-7 business days and is based on extensive production experience. Your production time may vary from sequence-to-sequence. International orders may require additional shipping time. Express Genes fee is based on the capacity of the chip at time of order submission and will fluctuate.

Yes, the maximum limit when configuring a project on eCommerce is 1152 Express Genes. If you plan on placing an order with more than 1152 Express Genes, please contact Customer Support for help submitting your order.

No, the advertised turnaround time is the amount of time it takes to manufacture Express Genes.

All Clonal Genes (Express & Standard) are shipped using overnight or priority service.

Typical shipping times by region are as follows: 

• North America: 1-2 business days
• Europe: 2-3 business days
• Asia: 2-4 business days
• South America: 3-6 business days

Express Genes ordered with glycerol stocks and Express Genes at the 10 μg - 100 μg and 100 μg - 1 mg DNA Scales (i.e. resuspended DNA) are shipped on dry ice, which may add one business day to the overall turnaround time.

General considerations that may delay shipping and delivery include:

• Issues with payment methods
• Country-specific import requirements
• Biosafety sequence screening

Complex sequences often require more production time to synthesize and clone, and therefore, are only available at the standard production speed.

No, new custom vectors will need to be onboarded prior to ordering Express Genes. New Custom Vector Onboarding will take approximately 2-3 weeks. For more information on Custom Vector Onboarding, please see the link to our FAQs for additional information.

The Express service is an upgraded service for Clonal Genes that provides a faster turnaround  time than our Standard service. See below for a detailed breakdown on the differences between the Standard and Express services:

We continue to offer Clonal Genes produced with our Standard speed of service, which has a 10-15 business day turnaround time.

Contracted pricing will be reflected in the base price of each gene, and the Express service fee is added as a separate line item relative to that contracted base price.

Express Genes service employs dynamic pricing to ensure availability at all times and reliable on-time delivery, especially during high-demand periods. Whenever there's a change in pricing, it will be reflected on our eCommerce platform.

Twist is committed to providing the best experience possible. In the event Express Genes are delivered late, the Express service fee will be discounted 100% and you will be invoiced at the Standard Clonal Gene price. Terms and conditions for the policy are here.

Additionally, we stand behind Express Genes with our Twist Satisfaction Policy. In the event that you are not satisfied with your Express Genes, please notify us within thirty (30) days of order placement.

All Clonal Gene sequences between 300bp to 5000bp that are scored as “Standard” complexity by our screener are eligible for the Express service.

Our scoring system adopts a machine learning model which analyzes and combines multiple sequence parameters ( i.e. overall GC percent, maximum homopolymer length, maximum repeat length, sequence length, repeat density, etc.). This model was trained using our historical manufacturing data, and it provides a more precise estimation of production success for genes and antibody products.

Complexity issues are driven mainly by repetitive structures and extreme GC content. Guidelines for sequence design are available online and below:

• Avoid repeats of ≥ 20bp or Tm ≥ 60C 
• Global GC content must be between 25% and 65%
• Avoid extreme differences in GC content within a gene (i.e. the difference in GC content between the highest and lowest 50 bp stretch should be no greater than 52%)
• Minimize homopolymers
• Minimize the number/length of small repeats scattered throughout the sequence
• For HIS tags, use a combination of CAC and CAT codons i.e. CACCAT

The likelihood of sequence acceptance improves by limiting repeats and reducing GC content. For example: a gene may score as “Not Accepted” with 66% overall GC content, but when reduced to 65% or less, may be accepted as a “Complex” sequence. Further lowering GC content to ~60% or less may improve the complexity score to “Standard”, at which point the sequence would be eligible for the Express service. The same holds true for eliminating repeats, reducing homopolymer stretches, and evening out extreme variations in GC windows. The more such structures can be avoided or reduced in a gene, the more likely it is to qualify for the Express service.

For additional information on our scoring algorithm and sequence design, please visit our FAQs.

Only “Standard” complexity sequences are eligible for the Express service. If a gene sequence is grayed out under the Express Genes section, that means it is a “Complex” or “Not accepted” sequence and will be marked as such in the eCommerce platform.

Express genes are available in the following DNA prep sizes: 1 μg - 4 μg, 4 μg - 10 μg, 10 μg - 100 μg and 100 μg - 1 mg. Turnaround time increases with prep size and is as follows: 4-7 business days for 1 μg - 4 μg and 4 μg - 10 μg, and 7-10 business days for 10 μg - 100 μg and 100 μg - 1 mg. The same shipment format options are available for Standard and Express genes.

Clonal Gene sequences scored as “Standard” complexity are eligible for the Express service. The Twist Bioscience eCommerce platform will score all sequences once they are uploaded and flag which ones are eligible for the Express service.

See here for additional information on sequence scoring parameters. 

This indicates that we have reached maximum capacity for the current Express Genes run. Express Genes are run multiple times a day, please check back soon.

Ordering Clonal Genes with our Express service ensures that an order receives top priority at every production stage. With additional resources dedicated to our new tier of service, this prioritized approach allows us to expedite the production of Clonal Genes without compromising quality.

Yes, a fee is added to each gene with the Express service applied. The fee will not be invoiced for any Express Gene that does not ship within the advertised turnaround time.

Express Genes are Clonal Genes produced faster using Twist’s Express service. The Express service reduces production time as follows without compromising quality at any stage.*

1 µg - 4 µg & 4 µg - 10 µg DNA Scale:

Standard: 10 business days

Express: 4-7 business days  

10 µg - 100 µg & 100 µg - 1mg DNA Scale:

Standard: 13 business days

Express: 7-10 business days

*Terms and Conditions: Advertised turnaround time for Express Clonal Genes is 4-7 business days and is based on extensive production experience. Your production time may vary from sequence-to-sequence. International orders may require additional shipping time. Express Genes fee is based on the capacity of the chip at time of order submission and will fluctuate.

Yes, the maximum limit when configuring a project on eCommerce is 1152 Express Genes. If you plan on placing an order with more than 1152 Express Genes, please contact Customer Support for help submitting your order.

No, the advertised turnaround time is the amount of time it takes to manufacture Express Genes.

All Clonal Genes (Express & Standard) are shipped using overnight or priority service.

Typical shipping times by region are as follows: 

• North America: 1-2 business days
• Europe: 2-3 business days
• Asia: 2-4 business days
• South America: 3-6 business days

Express Genes ordered with glycerol stocks and Express Genes at the 10 μg - 100 μg and 100 μg - 1 mg DNA Scales (i.e. resuspended DNA) are shipped on dry ice, which may add one business day to the overall turnaround time.

General considerations that may delay shipping and delivery include:

• Issues with payment methods
• Country-specific import requirements
• Biosafety sequence screening

Complex sequences often require more production time to synthesize and clone, and therefore, are only available at the standard production speed.

No, new custom vectors will need to be onboarded prior to ordering Express Genes. New Custom Vector Onboarding will take approximately 2-3 weeks. For more information on Custom Vector Onboarding, please see the link to our FAQs for additional information.

The Express service is an upgraded service for Clonal Genes that provides a faster turnaround  time than our Standard service. See below for a detailed breakdown on the differences between the Standard and Express services:

We continue to offer Clonal Genes produced with our Standard speed of service, which has a 10-15 business day turnaround time.

Contracted pricing will be reflected in the base price of each gene, and the Express service fee is added as a separate line item relative to that contracted base price.

Express Genes service employs dynamic pricing to ensure availability at all times and reliable on-time delivery, especially during high-demand periods. Whenever there's a change in pricing, it will be reflected on our eCommerce platform.

Twist is committed to providing the best experience possible. In the event Express Genes are delivered late, the Express service fee will be discounted 100% and you will be invoiced at the Standard Clonal Gene price. Terms and conditions for the policy are here.

Additionally, we stand behind Express Genes with our Twist Satisfaction Policy. In the event that you are not satisfied with your Express Genes, please notify us within thirty (30) days of order placement.

All Clonal Gene sequences between 300bp to 5000bp that are scored as “Standard” complexity by our screener are eligible for the Express service.

Our scoring system adopts a machine learning model which analyzes and combines multiple sequence parameters ( i.e. overall GC percent, maximum homopolymer length, maximum repeat length, sequence length, repeat density, etc.). This model was trained using our historical manufacturing data, and it provides a more precise estimation of production success for genes and antibody products.

Complexity issues are driven mainly by repetitive structures and extreme GC content. Guidelines for sequence design are available online and below:

• Avoid repeats of ≥ 20bp or Tm ≥ 60C 
• Global GC content must be between 25% and 65%
• Avoid extreme differences in GC content within a gene (i.e. the difference in GC content between the highest and lowest 50 bp stretch should be no greater than 52%)
• Minimize homopolymers
• Minimize the number/length of small repeats scattered throughout the sequence
• For HIS tags, use a combination of CAC and CAT codons i.e. CACCAT

The likelihood of sequence acceptance improves by limiting repeats and reducing GC content. For example: a gene may score as “Not Accepted” with 66% overall GC content, but when reduced to 65% or less, may be accepted as a “Complex” sequence. Further lowering GC content to ~60% or less may improve the complexity score to “Standard”, at which point the sequence would be eligible for the Express service. The same holds true for eliminating repeats, reducing homopolymer stretches, and evening out extreme variations in GC windows. The more such structures can be avoided or reduced in a gene, the more likely it is to qualify for the Express service.

For additional information on our scoring algorithm and sequence design, please visit our FAQs.

Only “Standard” complexity sequences are eligible for the Express service. If a gene sequence is grayed out under the Express Genes section, that means it is a “Complex” or “Not accepted” sequence and will be marked as such in the eCommerce platform.

Express genes are available in the following DNA prep sizes: 1 μg - 4 μg, 4 μg - 10 μg, 10 μg - 100 μg and 100 μg - 1 mg. Turnaround time increases with prep size and is as follows: 4-7 business days for 1 μg - 4 μg and 4 μg - 10 μg, and 7-10 business days for 10 μg - 100 μg and 100 μg - 1 mg. The same shipment format options are available for Standard and Express genes.

Clonal Gene sequences scored as “Standard” complexity are eligible for the Express service. The Twist Bioscience eCommerce platform will score all sequences once they are uploaded and flag which ones are eligible for the Express service.

See here for additional information on sequence scoring parameters.