OVERVIEW DATA ORDERING RESOURCES
OVERVIEW

The Twist Standard Hybridization Reagent Kit v2 is the gold standard for efficient and specific binding of panel probes to your regions of interest. Coupled with Twist’s Catalog or Custom Panels, this standard hybridization workflow provides the optimal conditions for a broad range of content.

Optimum Coverage
Optimum Coverage
Delivers excellent uniformity and increased on-target rates
Fully automatable
Cost-efficient, even coverage
Accommodates a Broad Range of Panels
Accommodates a Broad Range of Panels
Standardized conditions require no optimization
Compatible with fixed content, custom, or spike-ins

The Twist Standard Hybridization Reagent Kit v2 is the gold standard for efficient and specific binding of panel probes to your regions of interest. Coupled with Twist’s Catalog or Custom Panels, this standard hybridization workflow provides the optimal conditions for a broad range of content.

Optimum Coverage
Optimum Coverage
Delivers excellent uniformity and increased on-target rates
Fully automatable
Cost-efficient, even coverage
Accommodates a Broad Range of Panels
Accommodates a Broad Range of Panels
Standardized conditions require no optimization
Compatible with fixed content, custom, or spike-ins
DATA
Optimized Out of the Box

Coverage obtained with Twist Core Exome (top panels) and coverage with the Twist Core Exome + exon spike-ins. Both conditions provided optimal coverage without changes to the hybridization conditions using the standard Hybridization and Wash Kit.

Coverage obtained with Twist Core Exome and Exon Spike Ins
Maximize sequencing efficiency with even coverage

Even coverage throughout the target results in the most cost-efficient sequencing, and can increase sequencing throughput with higher multiplexing capabilities. Or use this extra sequencing flow cell space to get greater depth on the same number of samples.

Coverage Distribution
Optimized Out of the Box

Coverage obtained with Twist Core Exome (top panels) and coverage with the Twist Core Exome + exon spike-ins. Both conditions provided optimal coverage without changes to the hybridization conditions using the standard Hybridization and Wash Kit.

Coverage obtained with Twist Core Exome and Exon Spike Ins
Maximize sequencing efficiency with even coverage

Even coverage throughout the target results in the most cost-efficient sequencing, and can increase sequencing throughput with higher multiplexing capabilities. Or use this extra sequencing flow cell space to get greater depth on the same number of samples.

Coverage Distribution
ORDERING
Standard Hybridization v2 SKUs

104445

Twist Standard Hyb and Wash Kit v2, 2 Reactions

104446

Twist Standard Hyb and Wash Kit v2, 12 Reactions

104447

Twist Standard Hyb and Wash Kit v2, 96 Reactions

104133

Twist Standard Wash Buffers v2, 2 reactions

104135

Twist Standard Wash Buffers v2, 12 reactions

104137

Twist Standard Wash Buffers v2, 96 reactions
Standard Hybridization v1 SKUs

101279

Twist Hybridization and Wash Kit, 2 Reactions

104178

Twist Hybridization and Wash Kit with Amplification Mix, 12 Reactions

101025

Twist Hybridization and Wash Kit, 12 Reactions

104179

Twist Hybridization and Wash Kit with Amplification Mix, 96 Reactions

101026

Twist Hybridization and Wash Kit, 96 Reactions
Standard Hybridization v2 SKUs

104445

Twist Standard Hyb and Wash Kit v2, 2 Reactions

104446

Twist Standard Hyb and Wash Kit v2, 12 Reactions

104447

Twist Standard Hyb and Wash Kit v2, 96 Reactions

104133

Twist Standard Wash Buffers v2, 2 reactions

104135

Twist Standard Wash Buffers v2, 12 reactions

104137

Twist Standard Wash Buffers v2, 96 reactions
Standard Hybridization v1 SKUs

101279

Twist Hybridization and Wash Kit, 2 Reactions

104178

Twist Hybridization and Wash Kit with Amplification Mix, 12 Reactions

101025

Twist Hybridization and Wash Kit, 12 Reactions

104179

Twist Hybridization and Wash Kit with Amplification Mix, 96 Reactions

101026

Twist Hybridization and Wash Kit, 96 Reactions
RESOURCES
Optimized for a Broad Range of Panels
Optimized for a Broad Range of Panels
Optimized for a Broad Range of Panels