Comprehensive Cancer Variant Reference Standard for Liquid Biopsy Research
NGS-based liquid biopsy is a rapidly evolving application that requires accurate and precise reference standards. The Twist Pan-cancer Reference Standard is a high-quality, standardized control for use in the research and development of NGS-based liquid biopsy assays. This reference standard is ideal for establishing the analytical limit of detection (LoD) for specific cancer variants and as a control to track the quality of an NGS assay workflow and assess the fidelity of the assay process during research and development of such assays.
Traditional reference standards are limited in the number and variation of variants and typically use cell line-derived DNA which can carry unwanted sequence variations and variable fragment length. The Twist cfDNA Pan-cancer Reference Standard incorporates a large and diverse set of variants for research use. This reference standard utilizes accurate DNA synthesis of variants with precise control over the specific variant allele frequencies (VAF) to produce a reference standard which closely mimics the size distribution and fragmentation profile of cell-free and circulating tumor DNA enabling higher performance assay development.
This reference standard consists of synthetically designed variant sequences that mimic circulating tumor DNA (ctDNA), combined with background DNA that is derived from, and closely mimics, human-derived cell-free DNA (cfDNA). The ctDNA sequences are designed as a tiled pool of ~167 bp sequences that closely mimic natural ctDNA and cover 458 individual mutations with 132 clinically actionable variants across 84 genes associated with cancer.
*For research use only. Not for use in any diagnostic or clinical procedures.
Comprehensive Cancer Variant Reference Standard for Liquid Biopsy Research
NGS-based liquid biopsy is a rapidly evolving application that requires accurate and precise reference standards. The Twist Pan-cancer Reference Standard is a high-quality, standardized control for use in the research and development of NGS-based liquid biopsy assays. This reference standard is ideal for establishing the analytical limit of detection (LoD) for specific cancer variants and as a control to track the quality of an NGS assay workflow and assess the fidelity of the assay process during research and development of such assays.
Traditional reference standards are limited in the number and variation of variants and typically use cell line-derived DNA which can carry unwanted sequence variations and variable fragment length. The Twist cfDNA Pan-cancer Reference Standard incorporates a large and diverse set of variants for research use. This reference standard utilizes accurate DNA synthesis of variants with precise control over the specific variant allele frequencies (VAF) to produce a reference standard which closely mimics the size distribution and fragmentation profile of cell-free and circulating tumor DNA enabling higher performance assay development.
This reference standard consists of synthetically designed variant sequences that mimic circulating tumor DNA (ctDNA), combined with background DNA that is derived from, and closely mimics, human-derived cell-free DNA (cfDNA). The ctDNA sequences are designed as a tiled pool of ~167 bp sequences that closely mimic natural ctDNA and cover 458 individual mutations with 132 clinically actionable variants across 84 genes associated with cancer.
*For research use only. Not for use in any diagnostic or clinical procedures.
DNA analysis of the Twist cfDNA Pan-Cancer Reference Standard and competitors reference standards show that Twist more closely mimics native cell-free DNA providing a more true to life test result.

Post sequencing library size distribution comparison between Twist and competitors show how similar Twist mimics native cfDNA samples including the jagged leading peaks.

DNA analysis of the Twist cfDNA Pan-Cancer Reference Standard and competitors reference standards show that Twist more closely mimics native cell-free DNA providing a more true to life test result.

Post sequencing library size distribution comparison between Twist and competitors show how similar Twist mimics native cfDNA samples including the jagged leading peaks.

104549
*Twist cfDNA Pan-cancer reference standard set, 300 ng per tube104563
Twist cfDNA Pan-cancer reference standard VAF 0% (WT), 3ug104564
Twist cfDNA Pan-cancer reference standard VAF 0.1 %, 3ug104565
Twist cfDNA Pan-cancer reference standard VAF 0.25 %, 3ug104566
Twist cfDNA Pan-cancer reference standard VAF 0.5 %, 3ug104567
Twist cfDNA Pan-cancer reference standard VAF 1 %, 3ug104568
Twist cfDNA Pan-cancer reference standard VAF 2 %, 3ug104569
Twist cfDNA Pan-cancer reference standard VAF 5 %, 3ug104176
Twist Library Preparation Kit with amp mix, Mechanical Fragmentation, 16 Samples104177
Twist Library Preparation Kit with amp mix, Mechanical Fragmentation, 96 Samples*Kit includes 7 VAFs individually: 0% (WT), 0.1%, 0.25%, 0.5%, 1%, 2%, 5% at 300ng each tube
*For research use only. Not for use in any diagnostic or clinical procedures.
104549
*Twist cfDNA Pan-cancer reference standard set, 300 ng per tube104563
Twist cfDNA Pan-cancer reference standard VAF 0% (WT), 3ug104564
Twist cfDNA Pan-cancer reference standard VAF 0.1 %, 3ug104565
Twist cfDNA Pan-cancer reference standard VAF 0.25 %, 3ug104566
Twist cfDNA Pan-cancer reference standard VAF 0.5 %, 3ug104567
Twist cfDNA Pan-cancer reference standard VAF 1 %, 3ug104568
Twist cfDNA Pan-cancer reference standard VAF 2 %, 3ug104569
Twist cfDNA Pan-cancer reference standard VAF 5 %, 3ug104176
Twist Library Preparation Kit with amp mix, Mechanical Fragmentation, 16 Samples104177
Twist Library Preparation Kit with amp mix, Mechanical Fragmentation, 96 Samples*Kit includes 7 VAFs individually: 0% (WT), 0.1%, 0.25%, 0.5%, 1%, 2%, 5% at 300ng each tube
*For research use only. Not for use in any diagnostic or clinical procedures.
Data Files
Twist cfDNA Pan-Cancer Reference Standard Variant List: In .VCF and Excel format
Guide/Guideline
Twist cfDNA Pan-cancer Reference Standard Technical Guide For use with the Twist NGS Workflow
Data Files
Twist cfDNA Pan-Cancer Reference Standard Variant List: In .VCF and Excel format
Guide/Guideline
Twist cfDNA Pan-cancer Reference Standard Technical Guide For use with the Twist NGS Workflow