Performance at any scale
Our revolutionary silicon-based DNA Synthesis platform is capable of producing over a million unique ssDNA oligos in a single run. Each chip contains Thousands of discrete clusters each containing 121 individually addressable surfaces capable of synthesizing a unique oligo sequence. A representation of the silicon chip technology can be seen below.
Due to the sheer scale of DNA synthesis capability on the chip there are virtually no limitations to the number of unique oligos that can be assembled into a pool. Don't restrict the outcome of your application, order Oligo Pools tailored precisely to your experiment.
More Space for Innovation
Don’t be limited by length. Encode the elements and sequences you need, including promoters, enhancers, guides, and probes all within the expanded architecture. With oligo lengths up to 300nt it’s possible to encode multiple elements in a single design giving you the ability to do more with each oligo.
Screen once, screen right
Unmatched pool uniformity without bias provides more unique targets per screen. This means less effort is required to achieve the data coverage you need, saving time and money.
Be data confident
With the precision and uniformity of Twist Oligo Pools, you can be confident in the quality of your experimental results. Don’t miss a datapoint due to synthesis errors or inefficiencies. Get industry-leading oligo representation and low error rate for oligos of any length up to 300nt, and pools of any size. Leverage these exceptional quality oligo pools at the precise scale and length required for your application.
Learn how you can create multiple focused CRISPR libraries from a single Oligo Pool to expand your research and save time and money in the process.
Performance at any scale
Our revolutionary silicon-based DNA Synthesis platform is capable of producing over a million unique ssDNA oligos in a single run. Each chip contains Thousands of discrete clusters each containing 121 individually addressable surfaces capable of synthesizing a unique oligo sequence. A representation of the silicon chip technology can be seen below.
Due to the sheer scale of DNA synthesis capability on the chip there are virtually no limitations to the number of unique oligos that can be assembled into a pool. Don't restrict the outcome of your application, order Oligo Pools tailored precisely to your experiment.
More Space for Innovation
Don’t be limited by length. Encode the elements and sequences you need, including promoters, enhancers, guides, and probes all within the expanded architecture. With oligo lengths up to 300nt it’s possible to encode multiple elements in a single design giving you the ability to do more with each oligo.
Screen once, screen right
Unmatched pool uniformity without bias provides more unique targets per screen. This means less effort is required to achieve the data coverage you need, saving time and money.
Be data confident
With the precision and uniformity of Twist Oligo Pools, you can be confident in the quality of your experimental results. Don’t miss a datapoint due to synthesis errors or inefficiencies. Get industry-leading oligo representation and low error rate for oligos of any length up to 300nt, and pools of any size. Leverage these exceptional quality oligo pools at the precise scale and length required for your application.
Learn how you can create multiple focused CRISPR libraries from a single Oligo Pool to expand your research and save time and money in the process.
NGS quality control data from a typical oligo pool containing 23,000 90mer oligos show the uniformity of the pool at 300x read coverage. The corresponding table indicates the uniformity metrics for this pool. Twist oligos are synthesized bias-free with high uniformity and complete oligo representation.
Sequencing analysis of Oligo Pools generated by Twist Bioscience and an array-based competitor demonstrate that the Twist Oligo Pools contain 100% of the expected sequences and a higher percentage of correct sequences than the competitor pool.
NGS quality control data from a typical oligo pool containing 23,000 90mer oligos show the uniformity of the pool at 300x read coverage. The corresponding table indicates the uniformity metrics for this pool. Twist oligos are synthesized bias-free with high uniformity and complete oligo representation.
Sequencing analysis of Oligo Pools generated by Twist Bioscience and an array-based competitor demonstrate that the Twist Oligo Pools contain 100% of the expected sequences and a higher percentage of correct sequences than the competitor pool.
Guide RNA libraries for CRISPR screens
CRISPR screens involve the precise, high-throughput knock-out, interference or modulation of gene activity, allowing complex genotype-phenotype interactions to be unveiled. Oligo Pools are designed to encode multiple guide RNA molecules per gene.
Product benefits include:
- Full control over guide library design, avoiding unwanted guides, wasted sequencing, and limited guide-per-gene ratios.
- Pairing high-fidelity Cas enzymes with highly accurate synthesis for true high-fidelity screens with minimal off-target effects.
- Incredibly uniform, bias-free pools achieve amplified signal to noise ratio to achieve maximum hit detection, lowering downstream costs.
- Oligos up to 300nt make space for additional experimental elements. Add multiple guides per oligo, genome homology, or minimal promoters with ease.
Watch our webinar: Next generation CRISPR screens based on single-cell transcriptomics
Genome tiling libraries
Tiling libraries allow a comprehensive study of genomic functionality. Pools of oligos may encode peptide arrays, mRNA encoding sequences, or genome fragments.
Product benefits include:
- Tiling precise genomic regions of interest. Reduce experimental noise by ignoring unwanted sequences at the design step.
- Complex, repetitive or palindromic sequences that cannot typically be synthesized as genes can be encoded in oligos.
- Larger peptide structures or genome architectures can be encoded in oligos 300nt in length.
- Uniform synthesis minimizes oversampling required for comprehensive screening.
Read our article: Identifying Coronavirus Virulence Factors for RNA Vaccine Development with Twist Oligo Pools
High throughput genetic control screens
Understanding the genetic elements controlling gene expression and regulation has broad application in synthetic biology and development of potential disease treatment. Oligo Pools power high-throughput screens of genetic control, including gene expression profiling and reporter-based applications.
Product benefits include:
- High-quality pools ensure you can interrogate every base for functionality
- High uniformity means you screen less to find your answers
- Up to 300nt pools gives you the flexibility to add multiple elements or homology regions
Watch our webinar: Epitranscriptome Screening on TechNetworks
Fluorescence in-situ hybridization
Fluorescence in-situ hybridization (FISH) allows the abundance and spatial organization of nucleic acids in biological samples to be directly visualized. Oligo Pools can encode probes that when bound to fluorescent markers provide tunable, programmable visualization of large numbers of targets.
Product benefits include:
- Rapid FISH probe development
- Sequence control enables effective targeting
- Longer oligos enable more specific targeting
Watch our webinar: Rapid and efficient production of FISH probes using oligo pools with Nicola Crosetto
Guide RNA libraries for CRISPR screens
CRISPR screens involve the precise, high-throughput knock-out, interference or modulation of gene activity, allowing complex genotype-phenotype interactions to be unveiled. Oligo Pools are designed to encode multiple guide RNA molecules per gene.
Product benefits include:
- Full control over guide library design, avoiding unwanted guides, wasted sequencing, and limited guide-per-gene ratios.
- Pairing high-fidelity Cas enzymes with highly accurate synthesis for true high-fidelity screens with minimal off-target effects.
- Incredibly uniform, bias-free pools achieve amplified signal to noise ratio to achieve maximum hit detection, lowering downstream costs.
- Oligos up to 300nt make space for additional experimental elements. Add multiple guides per oligo, genome homology, or minimal promoters with ease.
Watch our webinar: Next generation CRISPR screens based on single-cell transcriptomics
Genome tiling libraries
Tiling libraries allow a comprehensive study of genomic functionality. Pools of oligos may encode peptide arrays, mRNA encoding sequences, or genome fragments.
Product benefits include:
- Tiling precise genomic regions of interest. Reduce experimental noise by ignoring unwanted sequences at the design step.
- Complex, repetitive or palindromic sequences that cannot typically be synthesized as genes can be encoded in oligos.
- Larger peptide structures or genome architectures can be encoded in oligos 300nt in length.
- Uniform synthesis minimizes oversampling required for comprehensive screening.
Read our article: Identifying Coronavirus Virulence Factors for RNA Vaccine Development with Twist Oligo Pools
High throughput genetic control screens
Understanding the genetic elements controlling gene expression and regulation has broad application in synthetic biology and development of potential disease treatment. Oligo Pools power high-throughput screens of genetic control, including gene expression profiling and reporter-based applications.
Product benefits include:
- High-quality pools ensure you can interrogate every base for functionality
- High uniformity means you screen less to find your answers
- Up to 300nt pools gives you the flexibility to add multiple elements or homology regions
Watch our webinar: Epitranscriptome Screening on TechNetworks
Fluorescence in-situ hybridization
Fluorescence in-situ hybridization (FISH) allows the abundance and spatial organization of nucleic acids in biological samples to be directly visualized. Oligo Pools can encode probes that when bound to fluorescent markers provide tunable, programmable visualization of large numbers of targets.
Product benefits include:
- Rapid FISH probe development
- Sequence control enables effective targeting
- Longer oligos enable more specific targeting
Watch our webinar: Rapid and efficient production of FISH probes using oligo pools with Nicola Crosetto
Oligo Length
Up to 300ntOligo Pool Size
No limits on pool sizeYield
>0.2 fmol of each oligoUniformity
>90% oligos represented within <2.5x of the meanError rate
Up to 1:2000Turn-Around Time
Starting from 5 business daysPrice
Industry-leading pricingOligo Length
Up to 300ntOligo Pool Size
No limits on pool sizeYield
>0.2 fmol of each oligoUniformity
>90% oligos represented within <2.5x of the meanError rate
Up to 1:2000Turn-Around Time
Starting from 5 business daysPrice
Industry-leading pricing
Webinar
Discovering PARP inhibitor resistance mechanisms using genome-wide and focused CRISPR screens
Protocol
These guidelines outline the best practices for resuspending Twist Gene Fragments, Clonal Genes, Oligo Pools, and Variant Libraries.
Webinar
Discovering PARP inhibitor resistance mechanisms using genome-wide and focused CRISPR screens
Protocol
These guidelines outline the best practices for resuspending Twist Gene Fragments, Clonal Genes, Oligo Pools, and Variant Libraries.
