Explore VHH sequence space with three new single domain antibody libraries.
VHH Ratio
Specific oligo pools model the natural VHH repertoire
VHH Shuffle
Natural llama CDR sequences in the context of a llama consensus framework
VHH hShuffle
Natural llama CDR sequences in the context of a partially humanized VHH framework
Explore VHH sequence space with three new single domain antibody libraries.
VHH Ratio
Specific oligo pools model the natural VHH repertoire
VHH Shuffle
Natural llama CDR sequences in the context of a llama consensus framework
VHH hShuffle
Natural llama CDR sequences in the context of a partially humanized VHH framework
- 2391 CDR sequences analyzed for position-specific variation
- Controlled CDR diversity introduced in the library
- Consensus llama framework
- Each unique CDR individually synthesized
- Shuffled in consensus llama framework
- Final diversity of library > theoretical diversity
- Theoretical library diversity of 3.2 × 10^9
- Shuffled CDRs with theoretical library diversity of 3.2 × 109
- Partially humanized framework: Framework 1, 3 and 4 were
humanized using the human germline DP-47 framework
- 2391 CDR sequences analyzed for position-specific variation
- Controlled CDR diversity introduced in the library
- Consensus llama framework
- Each unique CDR individually synthesized
- Shuffled in consensus llama framework
- Final diversity of library > theoretical diversity
- Theoretical library diversity of 3.2 × 10^9
- Shuffled CDRs with theoretical library diversity of 3.2 × 109
- Partially humanized framework: Framework 1, 3 and 4 were
humanized using the human germline DP-47 framework
1 × 384 well plate picked per library per round
Out of 140 VHH binders
- 51 variants < 100 nM
- 90 variants < 200 nM
Anti-TIGIT clones from VHH libraries encompass a range of affinities and diversity
1 × 384 well plate picked per library per round
Out of 140 VHH binders
- 51 variants < 100 nM
- 90 variants < 200 nM
Anti-TIGIT clones from VHH libraries encompass a range of affinities and diversity
