How are my antibodies inspected for quality?

Twist inspects every antibody product for yield (μg), purity (%), and size (kDa) under reducing conditions. Each antibody will be inspected via LabChip (CE-SDS) to inspect purity and size of the individual heavy and light chains. Yields are quantified using A280 readings. Supernatant samples are quantified using a titer determination method. In the finalized documentation, each purified antibody will have reported volume, yield, and concentration values in addition to percent purity and heavy chain and light chain sizing. Supernatant documentation will provide antibody titer expression levels of either low, medium or high expression levels.

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