Can I submit oligos to Twist with degenerate bases?
No, our DNA synthesizers can only synthesize sequences containing the four standard bases A, C, G and T. The use of degenerate bases (R, Y, S, W, K, M, B, D, H, V, N) are not allowed. However, if you would like to have all four bases (or a subset of bases) at a given position in an oligo, you can design separate oligos, each with a different base at the site of interest.
Degenerate bases not allowed:
| IUPAC | BASES |
|---|---|
| R | A or G |
| Y | C or T |
| S | G or C |
| W | A or T |
| K | G or T |
| M | A or C |
| B | C or G or T |
| D | A or G or T |
| H | A or C or T |
| V | A or C or G |
| N | any base |
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