Can I submit oligos to Twist with degenerate bases?
As of December 2025, Twist supports the use of the N degenerate base in Oligo Pools.
Customers may now incorporate N at specified positions within their oligo designs. At this time, other degenerate bases (R, Y, S, W, K, M, B, D, H, V) are not supported. If you wish to include a defined subset of bases at a specific position, this can be achieved by designing separate oligos, each containing a different base at the position of interest.
| IUPAC | BASES |
|---|---|
| R | A or G |
| Y | C or T |
| S | G or C |
| W | A or T |
| K | G or T |
| M | A or C |
| B | C or G or T |
| D | A or G or T |
| H | A or C or T |
| V | A or C or G |
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