How long will oligos last and how should they be stored?

1. Unmodified oligos are stable. For long-term storage (up to one year), freeze DNA at -20° C. For even longer storage, freeze DNA at -80° C.

2. If the oligos are stored in solution, avoid repeated freeze-thaw cycles as this process can lead to physical degradation of the oligo.

3. If numerous experiments are planned, aliquot the sample and store at -20°C.

4. Careful handling is recommended to avoid possible contamination with nucleases or bacteria.

5. Twist DNA resuspension guidelines can be found here.

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