Eliminate Bias and Undesirable Motifs
Eliminate Bias and Undesirable Motifs
Eliminate Bias and Undesirable Motifs
OVERVIEW APPLICATION ORDERING RESOURCES
Overview

Combinatorial Assembly Libraries

Partner with Twist Bioscience to identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR), chimeric antigen receptor (CAR), and multispecific VHH libraries all through our combinatorial assembly libraries.

Combinatorial Assembly Libraries utilize seamless assembly of multiple variant domains to rapidly create libraries that enable high-throughput screening and characterization of novel and known variants for therapeutic discovery.

Combinatorial Assembly Libraries

Utilizing your customized, user defined gene parts, Twist Bioscience will create highly uniform combinatorial assembly libraries that you can use to screen for and identify desired combinations of custom gene parts within TCR, CAR or antibody VHH/scFv domains. 

TCR Combinatorial Assembly Library With Twist 

With arrayed TCR libraries (figure 1a), each user-defined alpha and beta chains are uniquely paired with one another to mimic the natural hit repertoire, where as with combinatorial TCR libraries (figure 1b), the alpha and beta chains are shuffled together to create a library of 16 different combinations, allowing for a library of greater diversity to interrogate the variant space beyond the identified repertoire.

TCR Library

Combinatorial TCR Library Data

Combinatorial

Arrayed TCR Library Data

Under discovery of TCRs is driven by the screening of immune cell repertoires and the re-synthesis of large numbers of potential binders. Combinatorial Assembly Libraries can be leveraged to rapidly generate arrayed and pooled combinations of TCR beta and alpha chains for high throughput screening. With arrayed TCR libraries, the alpha and beta chains are shuffled together to create a library of greater diversity to integrate the variant space beyond the identified repertoire.


arrayed data

We will provide you with highly uniform screening libraries that are precise, user-defined combinations of gene fragments, which enable you to efficiently test comprehensive screening of the desired combinations. 

Highly Diverse Libraries
Highly Diverse Libraries
Each combinatorial assembly can be composed of up to 10,000 gene fragment combinations
Customize Your Libraries
Customize Your Libraries
TCR: insert user defined combinatorial variants across alpha and beta chains
CAR: insert user defined combinatorial variants across hinge, transmembrane, and signaling domains
High Quality
High Quality
Libraries are all NGS-verified libraries with > 90% of possible variants present within 10x of the mean
High Throughput
High Throughput
Libraries are designed at scale with diversity across multiple elements of the sequence
Flexible
Flexible
Libraries are designed at scale with diversity across multiple elements of the sequence
Have questions?

Tell us what you would like more information on. We’re here to help!

Contact Us

Combinatorial Assembly Libraries

Partner with Twist Bioscience to identify and develop novel advanced cell therapies through large-scale, highly diverse T-cell Receptor (TCR), chimeric antigen receptor (CAR), and multispecific VHH libraries all through our combinatorial assembly libraries.

Combinatorial Assembly Libraries utilize seamless assembly of multiple variant domains to rapidly create libraries that enable high-throughput screening and characterization of novel and known variants for therapeutic discovery.

Combinatorial Assembly Libraries

Utilizing your customized, user defined gene parts, Twist Bioscience will create highly uniform combinatorial assembly libraries that you can use to screen for and identify desired combinations of custom gene parts within TCR, CAR or antibody VHH/scFv domains. 

TCR Combinatorial Assembly Library With Twist 

With arrayed TCR libraries (figure 1a), each user-defined alpha and beta chains are uniquely paired with one another to mimic the natural hit repertoire, where as with combinatorial TCR libraries (figure 1b), the alpha and beta chains are shuffled together to create a library of 16 different combinations, allowing for a library of greater diversity to interrogate the variant space beyond the identified repertoire.

TCR Library

Combinatorial TCR Library Data

Combinatorial

Arrayed TCR Library Data

Under discovery of TCRs is driven by the screening of immune cell repertoires and the re-synthesis of large numbers of potential binders. Combinatorial Assembly Libraries can be leveraged to rapidly generate arrayed and pooled combinations of TCR beta and alpha chains for high throughput screening. With arrayed TCR libraries, the alpha and beta chains are shuffled together to create a library of greater diversity to integrate the variant space beyond the identified repertoire.


arrayed data

We will provide you with highly uniform screening libraries that are precise, user-defined combinations of gene fragments, which enable you to efficiently test comprehensive screening of the desired combinations. 

Highly Diverse Libraries
Highly Diverse Libraries
Each combinatorial assembly can be composed of up to 10,000 gene fragment combinations
Customize Your Libraries
Customize Your Libraries
TCR: insert user defined combinatorial variants across alpha and beta chains
CAR: insert user defined combinatorial variants across hinge, transmembrane, and signaling domains
High Quality
High Quality
Libraries are all NGS-verified libraries with > 90% of possible variants present within 10x of the mean
High Throughput
High Throughput
Libraries are designed at scale with diversity across multiple elements of the sequence
Flexible
Flexible
Libraries are designed at scale with diversity across multiple elements of the sequence
Have questions?

Tell us what you would like more information on. We’re here to help!

Contact Us

APPLICATION
T Cell Receptor (TCR) Discovery workflow using Combinatorial Assembly Libraries

Combinatorial Assembly Libraries can be leveraged to rapidly generate arrayed and pooled combinations of TCR beta and alpha chains for high throughput screening and the identification of TCRs against tumor neoantigens.

T Cell Receptor (TCR) Discovery workflow using Combinatorial Assembly Libraries
Chimeric antigen receptor (CAR) workflow using Combinatorial Assembly Libraries

Each domain of the CAR scaffold influences the functionality of the CAR both independently and synergistically. Combinatorial Assembly Libraries serve as a tool to fine-tune each of the modules to uncover codependencies and better understand the impact on T-cell specificity, antigen recognition and T-cell function. 

Chimeric antigen receptor (CAR) workflow using Combinatorial Assembly Libraries
When you partner with Twist Bioscience for custom, flexible libraries for TCR discovery and CAR T development you can expect:
  • Combinatorial libraries ready for screening that are a realistic dataset comprised of every variable domain possible and are precisely synthesized as a fragment 

  • Option to swap out scaffold components and test with ease

  • Ability to freely shuffle subunits to identify new arrangements that elicit precise and desirable immune responses

  • Process smaller component libraries (CARs) and screen against novel targets (TCRs)

  • NGS verified libraries so you know exactly which variants are being screened and can proceed to the next iteration of library design even faster

flexible libraries for TCR discovery and CAR T development

 

T Cell Receptor (TCR) Discovery workflow using Combinatorial Assembly Libraries

Combinatorial Assembly Libraries can be leveraged to rapidly generate arrayed and pooled combinations of TCR beta and alpha chains for high throughput screening and the identification of TCRs against tumor neoantigens.

T Cell Receptor (TCR) Discovery workflow using Combinatorial Assembly Libraries
Chimeric antigen receptor (CAR) workflow using Combinatorial Assembly Libraries

Each domain of the CAR scaffold influences the functionality of the CAR both independently and synergistically. Combinatorial Assembly Libraries serve as a tool to fine-tune each of the modules to uncover codependencies and better understand the impact on T-cell specificity, antigen recognition and T-cell function. 

Chimeric antigen receptor (CAR) workflow using Combinatorial Assembly Libraries
When you partner with Twist Bioscience for custom, flexible libraries for TCR discovery and CAR T development you can expect:
  • Combinatorial libraries ready for screening that are a realistic dataset comprised of every variable domain possible and are precisely synthesized as a fragment 

  • Option to swap out scaffold components and test with ease

  • Ability to freely shuffle subunits to identify new arrangements that elicit precise and desirable immune responses

  • Process smaller component libraries (CARs) and screen against novel targets (TCRs)

  • NGS verified libraries so you know exactly which variants are being screened and can proceed to the next iteration of library design even faster

flexible libraries for TCR discovery and CAR T development

 

Ordering

Visit our eCommerce platform to create or login to your Twist Bioscience account. Once you have logged into your account on the the eCommerce site, go to "libraires" and click on "Download Combinatorial Assembly Libraries Submission Form." Following, fill out the downloaded submission form and send it to  [email protected] for design review. Once the design review is complete, we will provide you with a quote. The price and turnaround time of your combinatorial assembly libraries will vary per project. 

Visit our eCommerce platform to create or login to your Twist Bioscience account. Once you have logged into your account on the the eCommerce site, go to "libraires" and click on "Download Combinatorial Assembly Libraries Submission Form." Following, fill out the downloaded submission form and send it to  [email protected] for design review. Once the design review is complete, we will provide you with a quote. The price and turnaround time of your combinatorial assembly libraries will vary per project. 

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