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Protocol

Mechanical Fragmentation and Twist Universal Adapter System: For use with the Twist NGS Workflow

This Twist Library Preparation Kit provides the reagents needed to prepare genomic DNA (gDNA) libraries using mechanically fragmented DNA and the Twist Universal Adapter System. The Twist Universal Adapter System consists of Twist Universal Adapters and Twist Unique Dual Indexed (UDI) Primers. This manual details the steps for generating the amplified, indexed libraries needed for downstream target enrichment and sequencing on Illumina next-generation sequencing (NGS) systems. This library preparation protocol is optimized for use with Twist Target Enrichment Kits and should only be performed with reagents specified or their equivalents.