How does Twist test the quality of the gene synthesis?
All of our Clonal Gene synthesis products are NGS sequence verified.
Gene Fragments are not NGS verified. With a low error rate for gene synthesis, we estimate that you will need to sequence approximately 3, 4, or 6 colonies for Gene Fragments of 600, 1200, or 1800 bps respectively to obtain a sequence perfect clone with 99% confidence.
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